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http://dx.doi.org/10.4014/mbl.2004.04009

Medium Optimization for Cell Growth and Metabolite Formation from Haematococcus sp. under Mixotrophic Cultivation  

Kim, Hyo Seon (Department of Biotechnology, Pukyong National University)
Kim, Sung-Koo (Department of Biotechnology, Pukyong National University)
Jeong, Gwi-Taek (Department of Biotechnology, Pukyong National University)
Publication Information
Microbiology and Biotechnology Letters / v.48, no.3, 2020 , pp. 337-343 More about this Journal
Abstract
In this study, the medium optimization for cell growth and metabolite formation of Haematococcus sp. under mixotrophic cultivation was investigated. As a result, modified MS medium was selected as the basal medium; glucose was selected as the carbon source, with an optimum concentration of 10 g/l, and potassium nitrate was chosen as the nitrogen source, with an optimum concentration of 1.9 g/l. Under optimum conditions, Haematococcus sp. demonstrated an increase in biomass concentration from 0.18 gDW/l to 5.58 gDW/l in 14 days, after which there was a 31-fold increase in its growth. At the same time, the concentrations of chlorophyll and carotenoids were 172.16 mg/l and 42.33 mg/l, respectively. This work will contribute to the basic data for mass cultivation of microalgae.
Keywords
Haematococcus sp.; mixotrophic cultivation; medium optimization; chlorophyll; carotenoids;
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