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http://dx.doi.org/10.4014/mbl.1912.12009

Probiotic Potential of Pediococcus acidilactici and Enterococcus faecium Isolated from Indigenous Yogurt and Raw Goat Milk  

Sarkar, Shovon Lal (Department of Microbiology, Jashore University of Science and Technology)
Hossain, Md. Iqbal (Department of Microbiology, Jashore University of Science and Technology)
Monika, Sharmin Akter (Department of Microbiology, Jashore University of Science and Technology)
Sanyal, Santonu Kumar (Department of Microbiology, Jashore University of Science and Technology)
Roy, Pravas Chandra (Department of Microbiology, Jashore University of Science and Technology)
Hossain, Md. Anwar (Department of Microbiology, University of Dhaka)
Jahid, Iqbal Kabir (Department of Microbiology, Jashore University of Science and Technology)
Publication Information
Microbiology and Biotechnology Letters / v.48, no.3, 2020 , pp. 276-286 More about this Journal
Abstract
Probiotics are live microorganisms that, when administered in adequate amounts, confer health benefits to the host. This study was conducted for the isolation of potential lactic acid bacteria (LAB) with probiotic properties from goat milk and yogurt. Several tests were conducted in vitro using the standard procedures for evaluating the inhibitory spectra of LAB against pathogenic bacteria; tolerance to NaCl, bile salt, and phenol; hemolytic, milk coagulation, and bile salt hydrolase activities; gastrointestinal transit tolerance; adhesion properties; and antibiotic susceptibility. Among 40 LAB strains screened according to culture characteristics, five isolates exhibited antagonistic properties. Three were identified as Pediococcus acidilactici, and two were identified as Enterococcus faecium, exploiting 16S rRNA gene sequencing. All the isolates succeeded in the gastrointestinal transit tolerance assay and successively colonized mucosal epithelial cells. Based on the results of these in vitro assays, both P. acidilactici and E. faecium can be considered as potential probiotic candidates.
Keywords
Probiotic; antagonistic assay; Pediococcus acidilactici; Enterococcus faecium; goat milk; 16S rRNA;
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1 Kolida S, Saulnier D, Gibson G. 2006. Gastrointestinal microflora: Probiotics. Adv. Appl. Microbiol. 59: 187-219.   DOI
2 Yadav R, Puniya A, Shukla P. 2016. Probiotic properties of Lactobacillus plantarum RYPR1 from an indigenous fermented beverage raabadi. Front. Microbiol. 7: 1683.   DOI
3 Yu B, Liu R, Hsiao F, Chiou P. 2008. Evaluation of Lactobacillus reuteri Pg4 strain expressing heterologous ${\beta}$-glucanase as a probiotic in a barley-base poultry diet. Animal. Feed. Sci. Technol. 141: 82-91.   DOI
4 Rajoka MR, Shi J, Zhu J, Shao D, Huang Q, Yang H, Jin M. 2017. Capacity of lactic acid bacteria in immunity enhancement and cancer prevention. App. Microbiol. Biotechnol. 101: 35-45.   DOI
5 Angmo K, Kumari A, Savitri, Bhalla TC. 2016. Probiotic characterization of lactic acid bacteria isolated from fermented foods and beverage of Ladakh. LWT - Food. Sci. Technol. 66: 428-435.   DOI
6 Larkin M, Blackshields G, Brown N, Chenna R, Mc-Gettigan P, Mc-William H, et al. 2007. Clustal W and Clustal X version 2.0. Bioinformatics (Oxford, England). 23: 2947-2948.   DOI
7 Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596-1599.   DOI
8 Tulumoglu S, Kaya H, Simsek O. 2014. Probiotic characteristics of Lactobacillus fermentum strains isolated from tulum cheese. Anaerobe 30: 120-125.   DOI
9 Ramos C, Thorsen L, Schwan R, Jespersen L. 2013. Strain-specific probiotics properties of Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus brevis isolates from Brazilian food products. Food. Microbiol. 36: 22-29.   DOI
10 Huang Y, Wang J, Wang F, Wu Y, Sui L, Yang, Wang Z. 2013. Lactobacillus plantarum strains as potential probiotic cultures with cholesterol-lowering activity. J. Dairy. Sci. 96: 2746-2753.   DOI
11 Flanagan R, Neal-McKinney J, Dhillon A, Miller W, Konkel M. 2009. Examination of Campylobacter jejuni putative adhesins leads to the identification of a new protein, designated FlpA, required for chicken colonization. Infect. Immun. 77: 2399-2407.   DOI
12 Chettri R, Tamang J. 2014. Functional properties of tungrymbai and bekang, naturally fermented soybean foods of North East India. Int. J. Ferm. Foods. 3: 87-103.   DOI
13 Abbasiliasi S, Shun TJ, Ibrahim T, Ramanan R, Vakhshiteh F, Mustafa S. 2012. Isolation of Pediococcus acidilactici Kp10 with ability to secrete bacteriocin-like inhibitory substance from milk products for applications in food industry. BMC. Microbiol. 12: 260.   DOI
14 Aswathy R, Ismail B, John P, Nampoothiri K. 2008. Evaluation of the probiotic characteristics of newly isolated lactic acid Bacteria. Appl. Biochem. Biotechnol. 10: 8183-8186.
15 de Moraes GMD, de Abreu LR, do Egito AS, Salles HO, da Silva LMF, Nero LA, et al. 2017. Functional Properties of Lactobacillus mucosae Strains Isolated from Brazilian Goat Milk. Prob. Antimicrob. Proteins. 9: 235-245.   DOI
16 Huang Y, Adams M. 2004. In vitro assessment of the upper gastrointestinal tolerance of potential probiotic dairy propionibacteria. Int. J. Food. Microbiol. 91: 253-260.   DOI
17 Vuyst LD, Moreno MF, Revets H. 2003. Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins. Int. J. Food. Microbiol. 84: 299-318.   DOI
18 Garcia V, Rovira S, Boutoial K, Lopez MB. 2014. Improvements in goat milk quality: A review. Small. Rumi. Res. 121: 51-57.   DOI
19 Argyri A, Zoumpopoulou G, Karatzas K, Tsakalidou E, Nychas G, Panagou E, et al. 2013. Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests. Food Microbiol. 33: 282-291.   DOI
20 FAO/WHO (Food and Agriculture Organization of the United Nations WHO. 2002. Joint FAO/WHO Working group report on drafting guidelines for the evaluation of probiotics in food London, Ontario, Canada.
21 Hoque MZ, Akter F, Hossain KM, Rahman MSM, Billah MM, Islam KMD. 2010. Isolation, identification and analysis of probiotic properties of Lactobacillus Spp. from selective regional yoghurts. World. J. Dairy. Food. Sci. 5: 39-46.
22 Erkkila S, Venalainen M, Hielm S, Petaja E, Puolanne E, Mattila-Sandholm T. 2000. Survival of Escherichia coli O157:H7 in dry sausage fermented by probiotic lactic acid bacteria. J. Sci. Food. Agri. 80: 2101-2104.   DOI
23 Pisano MB, Casula M, Corda A, Fadda ME, Deplano M, Cosentino S. 2008. In vitro probiotic characteristics of lactobacillus strains isolated from fiore sardo cheese. Ita. J. Food. Sci. 20: 505-516.
24 Bustos AY, Saavedra L, de Valdez GF, Raya RR, Taranto MP. 2012. Relationship between bile salt hydrolase activity, changes in the internal pH and tolerance to bile acids in lactic acid bacteria. Biotechnol. Lett. 34: 1511-1518.   DOI
25 Oh YJ, Jung DS. 2015. Evaluation of probiotic properties of Lactobacillus and Pediococcus strains isolated from Omegisool, a traditionally fermented milletalcoholic beverage in Korea. LWT. Food. Sci. Technol. 63: 437-444.   DOI
26 Coulibaly I, Robin Dubois D, Destain J, Philippe T. 2008. Characterization of lactic acid bacteria isolated from poultry farms in Senegal. Afr. J. Biotechnol. 7: 2006-2012.   DOI
27 Pundir RK, Rana S, Kashyap N, Kaur A. 2013. Probiotic potential of lactic acid bacteria isolated from food samples: an in vitro study. J. Appl. Pharm. Sci. 3: 85-93.
28 Ahmed T, Kanwal R, Ayub N. 2006. Influence of temperature on growth pattern of Lactococcus lactis, Streptococcus cremoris and Lactobacillus acidophilus isolated from camel milk. Biotechnology 5: 481-488.   DOI
29 Anadon A, Martinez-Larranaga MR, Aranzazu Martinez M. 2006. Probiotics for animal nutrition in the European Union. Regulation and safety assessment. Regul. Toxicol. Pharmacol. 45: 91-95.   DOI
30 Bhardwaj A, Kapila S, Mani J, Malik R. 2009. Comparison of susceptibility to opsonic killing by in vitro human immune response of Enterococcus strains isolated from dairy products, clinical samples and probiotic preparation. Int. J. Food. Microbiol. 128: 513-515.   DOI
31 Moroni O, Kheadr E, Boutin Y, Lacroix C, Fliss I. 2006. Inactivation of adhesion and invasion of food-borne Listeria monocytogenes by bacteriocin producin Bifidobacterium strains of human origin. Appl. Env. Microbiol. 72: 6894-6901.   DOI
32 Souza G, Ferrari S, Daiane C, Akmida L, Fatima K, Costa M, et al. 2013. Microbiological and physical-chemical profile of goat milk in the semiarid region of the San Francisco valley. Vet. Not. 19: 14-22.
33 Tripathi M, Giri S. 2014. Probiotic functional foods: Survival of probiotics during processing and storage. J. Funct. Foods. 9: 225-241.   DOI
34 Jayamanne V, Adams M. 2006. Determination of survival, identity, and stress resistance of probiotic bifidobacteria in bioyoghurts. Lett. Appl. Microbiol. 42: 189-194.   DOI
35 Braiek OB, Ghomrassi H, Cremonesi P, Morandi S, Fleury Y, Chevalier P, et al. 2017. Isolation and characterization of an enterocin Pproducing Enterococcus lactis strain from a fresh shrimp (Penaeus vannamei). Antonie. Van. Leeuwenhoek 110: 771-786.   DOI
36 M'hir S, Minervini F, Cagno RD, Chammem N, Hamdi M. 2011. Technological, functional and safety aspects of enterococci in fermented vegetable products: a mini-review. Ann. Microbiol. 62: 469-481.
37 Ben Braiek O, Ghomrassi H, Cremonesi P, Morandi S, Fleury Y, Le Chevalier P, et al. 2017. Isolation and characterisation of an enterocin P-producing Enterococcus lactis strain from a fresh shrimp (Penaeus vannamei). Antonie Van. Leeuwenhoek 110: 771-786.   DOI
38 Reuben, RC, Roy PC, Sarkar SL, Rubayet-Ul Alam, Jahid IK. 2019. Isolation, characterization, and assessment of lactic acid bacteria toward their selection as poultry probiotics. BMC Microbiol. 19: 253.   DOI
39 Mehta R, Arya R, Goyal K, Singh M, Sharma AK. 2013. Bio-preservative and therapeutic potential of pediocin: recent trends and future perspectives. Recent Pat. Biotechnol. 7: 172-178.   DOI