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http://dx.doi.org/10.4014/mbl.1909.09014

Carbon Storage Regulator A (csrA) Gene Regulates Motility and Growth of Bacillus licheniformis in the Presence of Hydrocarbons  

Angel, Laura Iztacihuatl Serrano (Laboratory of Molecular Microbiology and Environmental Biotechnology, Autonomous University of Guerrero)
Segura, Daniel (Department of Molecular Microbiology, Institute of Biotechnology, National Autonomous University of Mexico)
Jimenez, Jeiry Toribio (Laboratory of Molecular Microbiology and Environmental Biotechnology, Autonomous University of Guerrero)
Barrera, Miguel Angel Rodriguez (Laboratory of Molecular Microbiology and Environmental Biotechnology, Autonomous University of Guerrero)
Pineda, Carlos Ortuno (Laboratory of Nucleic Acids and Proteins, Autonomous University of Guerrero)
Ramirez, Yanet Romero (Laboratory of Molecular Microbiology and Environmental Biotechnology, Autonomous University of Guerrero)
Publication Information
Microbiology and Biotechnology Letters / v.48, no.2, 2020 , pp. 185-192 More about this Journal
Abstract
The global carbon storage regulator (Csr) system is conserved in bacteria and functions as a regulator in the exponential and stationary phases of growth in batch culture. The Csr system plays a role in the central carbon metabolism, virulence, motility, resistance to oxidative stress, and biofilm formation. Although the Csr was extensively studied in Gram negative bacteria, it has been reported only in the control of motility in Bacillus subtilis among Gram positive bacteria. The goal of this study was to explore the role of the csrA gene of Bacillus licheniformis M2-7 on motility and the bacterial ability to use hydrocarbons as carbon source. We deleted the csrA gene of B. licheniformis M2-7 using the plasmid pCsr-L, harboring the spectinomycin cassette obtained from the plasmid pHP45-omega2. Mutants were grown on culture medium supplemented with 2% glucose or 0.1% gasoline and motility was assessed by electron microscopy. We observed that CsrA negatively regulates motility by controlling the expression of the hag gene and the synthesis of flagellin. Notably, we showed the ability of B. licheniformis to use gasoline as a unique carbon source. Our results demonstrated that CsrA is an indispensable regulator for the growth of B. licheniformis M2-7 on gasoline.
Keywords
Csr system; regulation; Bacillus licheniformis; motility; growth; hydrocarbons;
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