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http://dx.doi.org/10.4014/mbl.1902.02006

Isolation of Xylitol-Producing Thermotolerant Yeast Millerozyma farinosa from Nuruk  

Jung, Eun-Hye (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University)
Bae, Young-Woo (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University)
Kwun, Se-Young (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University)
Park, Eun-Hee (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University)
Kim, Myoung-Dong (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University)
Publication Information
Microbiology and Biotechnology Letters / v.47, no.4, 2019 , pp. 530-535 More about this Journal
Abstract
Diverse types of nuruks (traditional Korean fermentation initiators) were examined in order to isolate thermotolerant yeast strains capable of utilizing xylose as a carbon source. Among twenty yeast strains that grew at 46℃, MBY/L1597 showed a notably higher specific growth rate than other strains. This strain was identified as Millerozyma farinosa. While the control strain M. farinosa KCTC27412 (= CBS7064) did not show xylose reductase (XR) activity and apparent growth at 46℃, M. farinosa MBY/L1597 exhibited XR activity of 4.98 ± 0.49 U/mg protein when NADPH was used as a cofactor. M. farinosa MBY/L1597 cultured at 46℃ produced (9.87 ± 1.00 g/l) xylitol from 20 g/l xylose, corresponding to approximately 50% yield. M. farinosa MBY/L1597 was deposited at the Korean Collection for Type Cultures as KCTC27797.
Keywords
Tolerance; Nuruk; xylose reductase; xylitol; yeast; Millerozyma farinosa;
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Times Cited By KSCI : 10  (Citation Analysis)
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