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http://dx.doi.org/10.4014/mbl.1904.04007

Inhibition of Nelumbo nucifera Stamens-derived Kaempferol on FcεRI-mediated GATA-1 Expression  

Shim, Sun-Yup (Department of Aqualife Science, College of Fisheries and Ocean Sciences, Chonnam National University)
Publication Information
Microbiology and Biotechnology Letters / v.47, no.3, 2019 , pp. 350-353 More about this Journal
Abstract
The transcription factor, GATA-1, plays an important role in the $Fc{\varepsilon}RI$ ${\alpha}$ chain expression in mast cells and basophils. This study was conducted to investigate the downregulation of the transcription factor GATA-1 by kaempferol isolated from Nelumbo nucifera stamens in $Fc{\varepsilon}RI$-mediated allergic reactions. Kaempferol inhibited $Fc{\varepsilon}RI$-mediated histamine release. Western blotting analysis and RT-PCR showed that the protein and mRNA expression of GATA-1 was suppressed by kaempferol in a dose-dependent manner. These results suggest that kaempferol may inactivate basophils by downregulating the $Fc{\varepsilon}RI$ ${\alpha}$ chain expression via the inhibition of the GATA-1 expression.
Keywords
Kaempferol; Nelumbo nucifera stamens; $Fc{\varepsilon}RI$; GATA-1; KU812F cells;
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