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http://dx.doi.org/10.4014/mbl.1809.09014

Nitrifying-genes Dynamics in the Enriched Bacterial Consortium Inoculated with Humic Soil  

Seo, Yoon-Joo (Department of Environmental Science and Engineering, Ewha Womans University)
Lee, Yun-Yeong (Department of Environmental Science and Engineering, Ewha Womans University)
Choi, Hyung-Joo (Department of Environmental Science and Engineering, Ewha Womans University)
Cho, Kyung-Suk (Department of Environmental Science and Engineering, Ewha Womans University)
Publication Information
Microbiology and Biotechnology Letters / v.47, no.2, 2019 , pp. 296-302 More about this Journal
Abstract
In this study, the effects of ammonium concentration ($117.5-1155.0mg-N{\cdot}l^{-1}$), nitrite concentration ($0-50.0mg-N{\cdot}l^{-1}$), and temperature ($15-35^{\circ}C$) on nitrification performance and its functional genes (amoA-arc, amoA-bac, hao) in an enriched consortium inoculated with humic acid were determined. Notably, the maximum nitrification rate value was observed at $315mg-N{\cdot}l^{-1}$ of ammonium, but the highest functional gene copy numbers were obtained at $630mg-N{\cdot}l^{-1}$ of ammonium. No inhibition of the nitrification rate and functional gene copy numbers was observed via the added nitrites. The optimum temperature for maximum nitrification performance was observed to be $30^{\circ}C$. The amoA-bac copy numbers were also greater than those of amoA-arc under all test conditions. Notably, amoA-arc copy numbers and nitrification efficiency showed a positive relationship in network analysis. These results indicate that ammonium-oxidizing archaea and bacteria play important roles in the nitrification process.
Keywords
Nitrification; functional gene; ammonium; nitrite; ammonium-oxidizing archaea; ammonium-oxidizing bacteria;
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