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http://dx.doi.org/10.4014/mbl.18012.12015

Metabolic Engineering of Corynebacterium glutamicum for N-acetylglucosamine Production  

Kim, Jin-Yeon (Department of Pharmacy, Kyungsung University)
Kim, Bu-yeon (Major in Food Biotechnology, School of Food Biotechnology & Nutrition, Kyungsung University)
Moon, Kyung-Ho (Department of Pharmacy, Kyungsung University)
Lee, Jin-Ho (Major in Food Biotechnology, School of Food Biotechnology & Nutrition, Kyungsung University)
Publication Information
Microbiology and Biotechnology Letters / v.47, no.1, 2019 , pp. 78-86 More about this Journal
Abstract
Recombinant Corynebacterium glutamicum producing N-acetylglucosamine (GlcNAc) was constructed by metabolic engineering. To construct a basal strain producing GlcNAc, the genes nagA, nagB, and nanE encoding N-acetylglucosamine-6-phosphate deacetylase, glucosamine-6-phosphate deaminase, and N-acetylmannosamine-6-phosphate epimerase, respectively, were sequentially deleted from C. glutamicum ATCC 13032, yielding strain KG208. In addition, the genes glmS and gna1 encoding glucosamine-6-phosphate synthase and glucosamine-6-phosphate N-acetyltransferase, which originated from C. glutamicum and Saccharomyces cerevisiae, respectively, were expressed in several expression vectors. Among several combinations of glmS and gna1 expression, recombinant cells expressing glmS and gna1 under control of the ilvC promoter produced 1.77 g/l of GlcNAc and 0.63 g/l of glucosamine in flask cultures.
Keywords
N-acetylglucosamine; glucosamine; Corynebacterium glutamicum; metabolic engineering;
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1 Hsieh JW, Wu HS, Wei YH, Wang SS. 2007. Determination and kinetics of producing glucosamine using fungi. Biotechnol. Prog. 23: 1009-1016.   DOI
2 Sitanggang AB, Sophia L, Wu HS. 2012. Aspects of glucosamine production using microorganisms. Int. Food Res. J. 19: 393-404.
3 Liu L, Liu Y, Shin HD, Chen RR, Li J, Du G, et al. 2013. Microbial production of glucosamine and N-acetylglucosamine: advances and perspectives. Appl. Microbiol. Biotechnol. 97: 6149-6158.   DOI
4 Nakamura M, Hikida M, Nakano T, Ito S, Hamano T, Kinoshita S. 1993. Characterization of water retentive properties of hyaluronan. Cornea 12: 433-436.   DOI
5 Park C, Chung KH, Jeong TR, Yang HP, Nam KS, Kim CH. 2000. Effects of N-acetylglucosamine on suppression of collagenolysis and bone resorption in mouse calvarial osteoblasts. J. Chitin Chitosan 5: 79-87.
6 Cohen-Kupiec R, Chet I. 1998. The Molecular biology of chitin digestion. Curr. Opin. Biotechnol. 9: 270-277.   DOI
7 Suresh PV. 2012. Biodegradation of shrimp processing biowaste and concomitant production of chitinase enzyme and N-acetyl-D-glucosamine by marine bacteria: production and process optimization. World J. Microbiol. Biotechnol. 28: 2945-2962.   DOI
8 Donzelli BGG, Ostroff G, Harman GE. 2003. Enhanced enzymatic hydrolysis of langostino shell chitin with mixtures of enzymes from bacterial and fungal sources. Carbohydr. Res. 338: 1823-1833.   DOI
9 Sitanggang AB, Wu HS, Wang SS, Ho YC. 2010. Effect of pellet size and stimulating factor on the glucosamine production using Aspergillus sp. BCRC 31742. Bioresour. Technol. 101: 3595-3601.   DOI
10 Nampoothiri KM, Sandhya TVBC, Sabu A, Szakacs G, Pandey A. 2004. Process optimization for antifungal chitinase production by Trichoderma harzianum. Process Biochem. 39: 1583-1590.   DOI
11 Zhang JX, Liu L, Li JH, Du GC, Chen J. 2012. Enhanced glucosamine production by Aspergillus sp. BCRC 31742 based on the time variant kinetics analysis of dissolved oxygen level. Bioresour. Technol. 111: 507-511.   DOI
12 Deng MD, Severson DK, Grund AD, Wassink SL, Burlingame RP, Berry A, et al. 2005. Metabolic engineering of Escherichia coli for industrial production of glucosamine and N-acetylglucosamine. Metab. Eng. 7: 201-214.   DOI
13 Liu Y, Liu L, Shin HD, Chen RR, Li J, Du G, et al. 2013. Pathway engineering of Bacillus subtilis for microbial production of Nacetylglucosamine. Metab. Eng. 19: 107-115.   DOI
14 Liu Y, Zhu Y, Li J, Shin HD, Chen RR, Du G, et al. 2014. Modular pathway engineering of Bacillus subtilis for improved N-acetylglucosamine production. Metab. Eng. 23: 42-52.   DOI
15 Becker J, Wittmann C. 2012. Bio-based production of chemicals, materials and fuels - Corynebacterium glutamicum as versatile cell factory. Curr. Opin. Biotechnol. 23: 631-640.   DOI
16 Syukur PH, Kang MS, Ferrer L, Han SS, Lee JY, Kim HS, et al. 2018. Rational engineering of the shikimate and related pathways in Corynebacterium glutamicum for 4-hydroxybenzoate production. J. Biotechnol. 282: 92-100.   DOI
17 Lee J. 2014. Development and characterization of expression vectors for Corynebacterium glutamicum. J. Microbiol. Biotechnol. 24: 70-79.   DOI
18 Schafer A, Tauch A, Jager W, Kalinowski J, Thierbach G, Puhler A. 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145: 69-73.   DOI
19 van der Rest ME, Lange C, Molenaar D. 1999. A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA. Appl. Microbiol. Biotechnol. 52: 541-545.   DOI
20 Matano C, Uhde A, Youn JW, Maeda T, Clermont L, Marin K, et al. 2014. Engineering of Corynebacterium glutamicum for growth and L-lysine and lycopene production from N-acetylglucosamine. Appl. Microbiol. Biotechnol. 98: 5633-5643.   DOI
21 Uhde A, Bruhl N, Goldbeck O, Matano C, Gurow O, Ruckert C, et al. 2016. Transcription of sialic acid catabolism genes in Corynebacterium glutamicum is subject to catabolite repression and control by the transcriptional repressor NanR. J. Bacteriol. 198: 2204-2218.   DOI
22 Mio T, Yamada-Okabe T, Arisawa M, Yamada-Okabe H. 1999. Saccharomyces cerevisiae GNA1, an essential gene encoding a novel acetyltransferase involved in UDP-N-acetylglucosamine synthesis. J. Biol. Chem. 274: 424-429.   DOI
23 Deng MD, Grund AD, Wassink SL, Peng SS, Nielsen KL, Huckins BD, et al. 2006. Directed evolution and characterization of Escherichia coli glucosamine synthase. Biochimie 88: 419-429.   DOI