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http://dx.doi.org/10.4014/mbl.1707.07003

Identification and Characterization of Agar-degrading Vibrio sp. GNUM08123 Isolated from Marine Red Macroalgae  

Chi, Won-Jae (Biological and Genetic Resource Assessment Division, National Institute of Biological Resource)
Kim, Yoon Hee (Department of Biological Science, Myongji University)
Kim, Jong-Hee (Department of Food and Nutrition, Seoil University)
Hong, Soon-Kwang (Department of Biological Science, Myongji University)
Publication Information
Microbiology and Biotechnology Letters / v.45, no.3, 2017 , pp. 243-249 More about this Journal
Abstract
An agar-degrading bacterium, designated as the GNUM08123 strain, was isolated from samples of red algae collected from the Yongil Bay near East Sea, Korea. The isolated GNUM08123 strain was gram-negative, aerobic, motile, and beige-pigmented, with $C_{16:0}$ (25.9%) and summed feature 3 (comprising $C_{16:1}{\omega}7c/iso-C_{15:0}2-OH$, 34.4%) as its major cellular fatty acids. A similarity search based on the 16S rRNA gene sequence revealed that it belonged to class Gammaproteobacteria and shared 97.7% similarity with the type strain Vibrio chagasii $R-3712^T$. The DNA G+C content of strain $GNUM08123^T$ was 46.9 mol%. The major isoprenoid quinone was ubiquinone-8. The results of DNA-DNA relatedness and 16S rRNA sequence similarity analyses, in addition to its phenotypic and chemotaxonomic characteristics, suggest that strain GNUM08123 is a novel species within genus Vibrio, designated as Vibrio sp. GNUM08123. Agarase production by strain GNUM08123 was induced by agar and sucrose, but was repressed probably owing to carbon catabolite repression by glucose and maltose.
Keywords
Agarase; Vibrio sp. GNUM08123; DSM25249; KCTC23757;
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