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http://dx.doi.org/10.4014/mbl.1611.11004

Properties of Antimicrobial Substances Produced by Bacillus species Isolated from Rice Straw  

Liu, Xiaoming (Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University)
Yao, Zhuang (Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University)
Shim, Jae Min (Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University)
Lee, Kang Wook (Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University)
Kim, Hyun-Jin (Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University)
Kim, Jeong Hwan (Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University)
Publication Information
Microbiology and Biotechnology Letters / v.45, no.2, 2017 , pp. 133-142 More about this Journal
Abstract
Two hundred bacilli were isolated from rice straw, and 3 strains showing strong inhibitory activities against Bacillus cereus ATCC14579 were selected for further analyses. RSC15 was identified as B. licheniforms, and RSC26 and RSC42 were identified as B. amyloliquefaciens by molecular identification methods. The inhibitory activities were heat stable, and half of the activity was retained for 20 min at $100^{\circ}C$. SDS-PAGE analyses of the culture supernatant indicated that 2 different kinds of antibacterial substances were present with sizes below 3.5 kDa. Antibacterial substances produced by B. licheniformis RSC15 were partially purified by column chromatography, and the specific activity increased from 955.0 AU/mg to 6,400 AU/mg.
Keywords
Bacillus licheniformis; Bacillus amyloliquefaciens; lipopeptides; antimicrobials;
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