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http://dx.doi.org/10.4014/mbl.1610.10002

Anti-Inflammatory Effect of Sargassum patens C. Agardh Ethanol Extract in LPS-induced RAW264.7 Cells and Mouse Ear Edema  

Kim, Min-Ji (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Min-Ju (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Park, Sun-Hee (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Choi, Hyeun-Deok (Institute of Fisheries Sciences, Pukyong National University)
Park, So-Yeong (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Ji-Hyun (Institute of Fisheries Sciences, Pukyong National University)
Jang, Mi-Ran (Health Functional Food Policy Division, Ministry of Food and Drug Safety)
Im, Moo-Hyeog (Department of Food Science and Biotechnology, Daegu University)
Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Publication Information
Microbiology and Biotechnology Letters / v.45, no.2, 2017 , pp. 110-117 More about this Journal
Abstract
The anti-inflammatory effect of Sargassum patens C. Agardh ethanol extract (SPEE) was examined based on the lipopolysaccharide (LPS)-induced inflammatory response in this study. SPEE treatment was not cytotoxic to macrophages compared to the control. The production of NO was suppressed by SPEE by approximately 28% at $100{\mu}g/ml$, and levels of interleukin-6, tumor necrosis $factor-{\alpha}$, and $interleukin-1{\beta}$ decreased in a dose-dependent manner. In addition, the expression of inducible nitric oxide synthase, cyclooxygenase-2, and nuclear $factor-{\kappa}B$ was suppressed by SPEE treatment. In vivo, croton oil-induced mouse ear edema was attenuated by SPEE and the infiltration of mast cells into the tissue decreased. Based on these results, SPEE inhibits the release of LPS-induced pro-inflammatory cytokines and mediators, suggesting that SPEE is a potential agent for anti-inflammatory therapies.
Keywords
Sargassum patens C. Agardh; anti-inflammation; nuclear factor kappa B; mitogen-activated protein kinases; ear edema;
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