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http://dx.doi.org/10.4014/mbl.1607.07008

Transcriptional Analysis of Genes Involved in Ectopic Sporulation in Streptomyces griseus  

Chi, Won-Jae (Biological and Genetic Resources Assessment Division, National Institute of Biological Resource)
Publication Information
Microbiology and Biotechnology Letters / v.44, no.4, 2016 , pp. 563-570 More about this Journal
Abstract
Two Streptomyces griseus strains, a wild-type strain and an A-factor-dependent transcriptional activator mutant strain harboring multiple copies of a gene, dasA, that encodes a substrate-binding protein of the ATP-binding cassette transporter, showed severe ectopic sporulation of young substrate hyphae in response to glucose. The effect of dasA overexpression on the ectopic sporulation of Streptomyces strains was evaluated by comparing the transcriptomes of the strain harboring multiple copies of dasA and a strain harboring empty vector. By DNA microarray, 4 genes (SGR794, SGR2469, SGR3656, and SGR3657) and 3 clusters (SGR795-797, SGR2377-2378, and SGR6997-6998) were differentially expressed by more than 2-fold in S. griseus strains harboring dasA. The DNA microarray result was validated by low-resolution S1 nuclease mapping.
Keywords
Streptomyces griseus; ectopic sporulation; DNA microarray; low resolution S1 nuclease mapping; RT-PCR;
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