Browse > Article
http://dx.doi.org/10.4014/mbl.1512.12013

Induction of p53-dependent Apoptosis by Resveratrol in Human Cancer Cells, A549 and SKOV3  

Lee, Seul Gi (Department of Food Engineering, Kyungpook National University)
Nam, Ju-Ock (Department of Food Engineering, Kyungpook National University)
Publication Information
Microbiology and Biotechnology Letters / v.44, no.2, 2016 , pp. 194-200 More about this Journal
Abstract
Resveratrol, a polyphenolic compound present in many fruits and vegetables such as grapes, mulberries, and peanuts, has been reported to have various biological effects. However, the molecular mechanisms underlying resveratrol-induced apoptosis in A549 ovarian cancer cells are not well understood. In this study, we investigated the effect of resveratrol on A549 lung cancer cells (expressing wild-type p53) and compared it with that observed for SKOV3 ovarian cancer cells (expressing null-type p53). Resveratrol significantly inhibited the viability and proliferation of A549 cells in a concentration- and time-dependent manner, compared with its effects on SKOV3 cells. It also induced A549 cell apoptosis, but did not affect anoikis resistance. Furthermore, the viability and proliferation of p53-knockdown A549 cells were unaffected by the presence of resveratrol. Therefore, we demonstrate that the anticancer effect of resveratrol against A549 lung cancer cells is dependent on the presence of functional p53.
Keywords
Resveratrol; apoptosis; anoikis; p53; A549; SKOV3;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Park H-J, Jin S, Oh YN, Kim BW, Kwon HJ. 2015. Induction of apoptosis by methanol extract of endlicheria anomala in human lung and liver cancer cells. J. Life Sci. 25: 441−449.   DOI
2 Kim BW, Kwon HJ, Jin K-S, Park H-J, Lee J-Y, Oh YN, et al. 2013. Induction of G2/M arrest and apoptosis by the methanol extract of typha orientalis in human colon adenocarcinoma HT29 cells. Korean J. Microbiol. Biotechnol. 41: 425−432.
3 Kim HH, Min G. 2015. Inhibitory effects of S-allylcysteine on cell proliferation of human cervical cancer cell line, HeLa. J. Life Sci. 25: 397−405.   DOI
4 Lee SH, Park SY, Kim I-S, Park OJ, Kim YM. 2012. Effects of resveratrol on migration and proliferation in HT-29 colon cancer cells. KSBB J. 27: 289−294.   DOI
5 Mukhopadhyay T, Roth JA. 1997. Induction of apoptosis in human lung cancer cells after wild-type p53 activation by methoxyestradiol. Oncogene 14: 379−384.   DOI
6 NaveenKumar SK, Thushara RM, Sundaram MS, Hemshekhar M, Paul M, Thirunavukkarasu C, et al. 2015. Unconjugated bilirubin exerts pro-apoptotic effect on platelets via p38-MAPK activation. Sci. Rep. 5: 15045.   DOI
7 Opipari AW Jr, Tan L, Boitano AE, Sorenson DR, Aurora A, Liu JR. 2004. Resveratrol-induced autophagocytosis in ovarian cancer cells. Cancer Res. 64: 696−703.   DOI
8 Park S-Y, Kim E-J, Lim D-Y, Kim J-S, Lim S-S, Shin H-K, et al. 2008. Inhibitory effect of the hexane extract of Saussurea lappa on the growth of LNCaP human prostate cancer cells. J. Korean Soc. Food Sci. Nutr. 37: 8−15.   DOI
9 Wang X, Lin G, Martins-Taylor K, Zeng H, Xu RH. 2009. Inhibition of caspase-mediated anoikis is critical for basic fibroblast growth factor-sustained culture of human pluripotent stem cells. J. Biol. Chem. 284: 34054−34064.   DOI
10 Wang H, Zhang H, Tang L, Chen H, Wu C, Zhao M, et al. 2013. Resveratrol inhibits TGF-beta1-induced epithelial-to-mesenchymal transition and suppresses lung cancer invasion and metastasis. Toxicology 303: 139−146.   DOI
11 Yoo J, Park S, Kang CS, Kang SJ, Kim BK. 2002. Expression of E-cadherin and p53 proteins in human soft tissue sarcomas. Arch Pathol. Lab. Med. 126: 33−38.
12 Hsieh TC, Juan G, Darzynkiewicz Z, Wu JM. 1999. Resveratrol increases nitric oxide synthase, induces accumulation of p53 and p21(WAF1/CIP1), and suppresses cultured bovine pulmonary artery endothelial cell proliferation by perturbing progression through S and G2. Cancer Res. 59: 2596−2601.
13 Chen J, Peng H, Ou-Yang X, He X. 2008. Research on the antitumor effect of ginsenoside Rg3 in B16 melanoma cells. Melanoma Res. 18: 322−329.   DOI
14 Ferraz da Costa DC, Casanova FA, Quarti J, Malheiros MS, Sanches D, Dos Santos PS, et al. 2012. Transient transfection of a wild-type p53 gene triggers resveratrol-induced apoptosis in cancer cells. PLoS One 7: e48746.   DOI
15 Fiucci G, Ravid D, Reich R, Liscovitch M. 2002. Caveolin-1 inhibits anchorage-independent growth, anoikis and invasiveness in MCF-7 human breast cancer cells. Oncogene 21: 2365−2375.   DOI
16 Kang SW. 2013. Role of reactive oxygen species in cell death pathways. Hanyang Med. Rev. 33: 77−82.   DOI
17 Min SO, Lee SW, Bak SY, Kim KS. 2015. Sphere-forming cultivation of liver cancer cell line in culture media without growth factors. Korean J. Clinical Oncol. 11: 20−23.   DOI
18 Corney DC, Flesken-Nikitin A, Godwin AK, Wang W, Nikitin AY. 2007. MicroRNA-34b and MicroRNA-34c are targets of p53 and cooperate in control of cell proliferation and adhesion-independent growth. Cancer Res. 67: 8433−8438.   DOI