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http://dx.doi.org/10.4014/mbl.1511.11007

Isolation of a New Agar Degrading Bacterium, Maribacter sp. SH-1 and Characterization of its Agarase  

Lee, Chang-Eun (Department of Green-Chemistry Convergence Engineering, Graduate School, Silla University)
Lee, Sol-Ji (Department of Bioscience, Graduate School, Silla University)
Lee, Dong-Geun (Department of Bioscience, Graduate School, Silla University)
Lee, Sang-Hyeon (Department of Green-Chemistry Convergence Engineering, Graduate School, Silla University)
Publication Information
Microbiology and Biotechnology Letters / v.44, no.2, 2016 , pp. 156-162 More about this Journal
Abstract
In this study, we isolated a new agar-degrading marine bacterium and characterized its agarase. An agardegrading marine bacterium SH-1 was isolated from seawater, collected from the seashore of Namhae in Gyeongnam province, Korea, and cultured in marine agar 2216 media. It was identified as Maribacter. sp. SH-1 by phylogenetic analyses, based on 16S rRNA gene sequence. The extracellular agarase was extracted from culture media of Maribacter sp. SH-1 and characterized. Its relative activities were 56, 62, 94, 100, and 8% at 20, 30, 40, 50, and 60℃, respectively, whereas 15, 100, 60, and 21% relative activities were observed at pH 5, 6, 7, and 8, respectively. Its extracellular agarase exhibited maximum activity (231 units/l) at pH 6.0 and 50℃, in 20 mM Tris-HCl buffer. Therefore, this agarase would be applicable as it showed the maximum activity at the temperature at which the agar is in a sol state. Furthermore, the agarase activities remained over 90% at 20, 30, and 40℃ after 0.5 h exposure at these temperatures. Thin layer chromatography analysis suggested that Maribacter sp. SH-1 produces extracellular β-agarase, as it hydrolyzes agarose to produce neoagarooligosaccharides, such as neoagarohexaose (34.8%), neoagarotetraose (52.2%), and neoagarobiose (13.0%). Maribacter sp. SH-1 and its β-agarase would be useful for the production of neoagarooligosaccharides, which shows functional properties, like skin moisturizing, skin whitening, inhibition of bacterial growth, and delay in starch degradation.
Keywords
Agar-degrading bacterium; β -agarase; marine bacterium; Maribacter sp. SH-1; neoagarooligosaccharides;
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