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http://dx.doi.org/10.4014/mbl.1512.12007

Cloning of Chondroitinase ABC from Bacteroides stercoris HJ-15, a Human Intestinal Anaerobic Bacterium  

Bang, Seo-Hyeon (College of Pharmacy, Kyung Hee University)
Shim, Juwon (College of Pharmacy, Kyung Hee University)
Hyun, Yang-Jin (College of Pharmacy, Kyung Hee University)
Kim, Dong-Hyun (College of Pharmacy, Kyung Hee University)
Publication Information
Microbiology and Biotechnology Letters / v.44, no.2, 2016 , pp. 140-144 More about this Journal
Abstract
The gene encoding chondroitinase ABC from a genomic library of Bacteroides stercoris HJ-15, which was isolated from human feces, was cloned. The cloned gene consisted of 3,090 bp and was predicted to encode a 1,029−amino-acid protein. The B. stercoris chondroitinase ABC gene was not homologous to other chondroitinase ABC genes; however, its amino acid sequence showed 71% homology to that of Bacteroides thetaiotaomicron. The gene was cloned in the pET-26b+ expression vector and expressed under the T7 promoter in Escherichia coli BL21(DE3). The purified recombinant chondroitinase ABC degraded chondroitin sulfates A, B, and C.
Keywords
Chondroitinase ABC; Bacteroides stercoris HJ-15; chondroitin sulfate; cloning;
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