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http://dx.doi.org/10.4014/kjmb.1311.11004

Optimization and Characterization of Conditions for Cellulose-degrading Crude Enzymes Produced by Cellulophaga lytica PKA 1005  

Bark, Si-Woo (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences, Pukyong National University)
Kim, Min-Ji (Institute of Fisheries Sciences, Pukyong National University)
Kang, Bo-Kyeong (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Pak, Won-Min (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Bo-Ram (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Park, Hong-Min (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Lim, Sung-Mee (Department of Food Nutrition and Science, Tongmyong University)
Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Publication Information
Microbiology and Biotechnology Letters / v.42, no.1, 2014 , pp. 18-24 More about this Journal
Abstract
This study was conducted to investigate optimum conditions for the production of cellulose-degrading crude enzymes by an isolated marine bacterium. A marine microorganism producing an extracellular cellulose-degrading enzyme was isolated from the red seaweed, Grateloupia elliptica Holmes. The isolated bacterium was identified as Cellulophaga lytica by 16S ribosomal RNA gene sequence analysis and physiological profiling and designated as Cellulophaga lytica PKA 1005. The optimum conditions for the growth of Cellulophaga lytica PKA 1005 were pH 7, 2% NaCl, and $30^{\circ}C$ with 36 h incubation time. To obtain the crude enzyme, the culture medium of the strain was centrifuged for 30 min at $12,000{\times}g$ and $4^{\circ}C$, and the supernatant was used as crude enzyme. The optimum conditions for the production of the cellulose-degrading crude enzyme were pH 8, $35^{\circ}C$, 8% carboxyl methyl cellulose, and 60 h reaction time.
Keywords
Cellulophaga lytica; cellulose-degrading crude enzyme;
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