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http://dx.doi.org/10.4014/kjmb.1308.08002

Induction of G2/M Arrest and Apoptosis by the Methanol Extract of Typha orientalis in Human Colon Adenocarcinoma HT29 Cells  

Jin, Soojung (Blue-Bio Industry RIC, Dong-Eui University)
Yun, Seung-Geun (Department of Life Science and Biotechnology, College of Natural Sciences, Dong-Eui University)
Oh, You Na (Blue-Bio Industry RIC, Dong-Eui University)
Lee, Ji-Young (Blue-Bio Industry RIC, Dong-Eui University)
Park, Hyun-Jin (Blue-Bio Industry RIC, Dong-Eui University)
Jin, Kyong-Suk (Blue-Bio Industry RIC, Dong-Eui University)
Kwon, Hyun Ju (Blue-Bio Industry RIC, Dong-Eui University)
Kim, Byung Woo (Blue-Bio Industry RIC, Dong-Eui University)
Publication Information
Microbiology and Biotechnology Letters / v.41, no.4, 2013 , pp. 425-432 More about this Journal
Abstract
Typha orientalis, also known as bulrush or cattail, is a perennial herbaceous plant found in freshwater wetlands and has been widely used in constructed wetlands for wastewater treatment. Recent data has revealed that SH21B, a mixture composed of seven herbs including T. orientalis, exhibited an anti-adipogenic activity by the inhibition of the expression of adipogenic regulators. However, the anti-cancer effect of T. orientalis and its molecular mechanisms remain unclear. In this study, we evaluated the anti-cancer effect and its mechanism in the methanol extract of T. orientalis (METO) on human colon carcinoma HT29 cells. It was found that METO treatment showed cytotoxic activity in a dose-dependent manner, and induced G2/M cell cycle arrest and apoptosis in HT29 cells. The induction of G2/M arrest by METO was associated with the up-regulation of phospho-Cdc2 (Tyr15), an inactive form of Cdc2 and the down-regulation of Cdc25c phosphatase. METO also induced tumor suppressor p53 and cyclin-dependent kinase inhibitor p21 (WAF1/CIP1) expression. In addition, METO-induced apoptosis was characterized by the proteolytic activation of caspase-3, degradation of poly ADP ribose polymerase (PARP), and up-regulation of death receptor FAS and pro-apoptotic Bax expression. Collectively, these results indicate that the cell cycle inhibition and apoptosis induction of METO in HT29 cells allows for the possibility of its use in anti-cancer therapies.
Keywords
Typha orientalis; anti-cancer effect; G2/M arrest; apoptosis;
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