Browse > Article
http://dx.doi.org/10.4014/kjmb.1307.07001

A Comparison of the Components and Biological Activities of the Ethanol Extracts of Achyranthes japonica Nakai and Achyranthes bidentata Blume  

Lee, Ye-Seol (Dept. of Food and Nutrition, Andong National University)
Kim, Mi-Sun (Dept. of Food and Nutrition, Andong National University)
Kim, Duck-Jin (Dept. of Food and Nutrition, Andong National University)
Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
Publication Information
Microbiology and Biotechnology Letters / v.41, no.4, 2013 , pp. 416-424 More about this Journal
Abstract
In the course of a study for the development of functional foods utilizing Woosul (Achyranthis radix), the components and various biological activities of Korean Woosul (AJN: Achyranthes japonica Nakai) and Chinese Woosul (ABB: Achyranthes bidentata Blume) were compared. Woosul in Korea, including AJN and ABB, are regulated and part of the Korean Pharmacopoeia. From AJN and ABB, ethanol extracts and their subsequent organic solvent fractions were prepared and their in-vitro antimicrobial, antioxidant and anti-diabetes activities were evaluated. Although AJN and ABB have no clear distinction in terms of usage in Korea, our results suggest that AJN has higher quantities of lipid-soluble components and lower amounts of water-soluble sugars than does ABB. ABB also appears to possess greater amounts of flavonoid and polyphenol substances than AJN. Analyses of biological activities showed that the fractions of AJN were more active as antibacterial agents, and possessed more pronounced ${\alpha}$-glucosidase inhibitory activities than those of the ABB fractions. However, the antioxidant activities of the ABB fractions, as determined by DPPH anion-, ABTS cation-, nitrite-scavenging activity and reducing power, were higher than those of the AJN fractions. Our results suggest that the components and bioactivity of the extracts and subsequent fractions of AJN and ABB are different. Therefore, usage of either AJN or ABB should be carefully considered, as regards their individual properties, when the active fractions of Woosul are employed in the development of functional foods or novel plant-derived medicines.
Keywords
Bioactivity; Achyranthes japonica Nakai; Achyranthes bidentata Blume;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Lim CS, Li CY, Kim YM, Lee WY, Rhee HI. 2005. The inhibitory effect of Corus walteri extract against ${\alpha}$-amylase. J. Korean Soc. Appl. Biol. Chem. 48: 103-108.
2 Oh TW, Park KH, Lee MY, Choi G, Park YK. 2012. Effects of the water extracts from Achyranthes radix on serum-deprivation-induced apoptosis in PC12 cells and transient cerebral middle artery occlusion-induced ischemic brains of rats. Korean J. Herbology 27: 77-83.
3 Park JS, Seong NS, Lee YJ. 2007. Comparative study on the anti-oxidative effects of Achyranthes japonica radix, Achyranthes bidentata radix, and Cyathula radix. Korean J. Herbology 22: 155-167.
4 Singleton VL, Orthofer, Lamuela-Raventos RRM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocaleau reagent. Methods Enzymol. 299: 152-178.   DOI
5 Sohn HY, Ryu HY, Jang YJ, Jang HS, Park YM, Kim SY. 2008. Evaluation of antimicrobial, antithrombin, and antioxidant activity of aerial part of Saxifraga stolonifera. Korean J. Microbiol. Biotechnol. 36: 195-200.
6 Valentina U, Fabcic J, Stampar F. 2007. Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.). Food Chem. 107: 185-192.
7 Xie F, Li X, Sun K, Chu Y, Cao H, Chen N, et al. 2001. An experimental study on drugs for improving blood circulation and removing blood stasis in treating mild chronic hepatic damages. J. Tradit. Chin. Med. 21: 225-231.
8 Yang L, Jiang H, Wang QH, Yang BY, Kuang HX. 2012. A new feruloyl tyramine glycoside from the roots of Achyranthes bidentata. Chin. J. Nat. Med. 10: 16-19.   DOI
9 Zhao BT, Jeong SY, Moon DC, Son KH, Son JK, Woo MH. 2012. High performance liquid chromatography used for quality control of Achyranthis radix. Arch. Pharm Res. 35: 1449-1455.   DOI   ScienceOn
10 Yoon HN, Yoon YK, Jang KH, Kim HW, Lee KS, Lee JS, et al. 2009. Antioxidant and anticancer activities by different parts in Achyranthes japonica and Achyranthes bidentata. Korean J. Medicinal Crop Sci. May, 7: 385-386.
11 Zhu X, Pan Y, Zheng L, Cui L, Cao Y. 2012. Polysaccharides from the Chinese medicinal herb Achyranthes bidentata enhance anti-malarial immunity during Plasmodium yoelii 17XL infection in mice. Malar. J. 11: 49-56.   DOI   ScienceOn
12 Kim HK, Jeung J, Park SJ, Kang SH, Song YS, Lee KN. 2004. Effects of extract of radix Achyranthis bidentatae on cadmium inhalation toxicity in rats. Korean J. Oriental Physiol. Pathol. 18: 274-283.
13 Kim KA, Lee JS, Park HJ, Kim JW, Kim CJ, Shim IS, et al. 2003. The inhibitory effect of Achyranthes bidentata radix extracts on cytochrome P450-catalyzed reactions in human liver microsomes. Korean J. Oriental Med. 24: 40-46.
14 Kim JH, Ki JY, Ann JY, Park HJ, Kim HJ, Kwak HB, et al. 2010. Inhibitory effects of Achyranthis bidentatae radix on osteoclast differentiation and bone resorption. Korean J. Herbology 25: 65-74.
15 Kim JI, Jang HS, Kim JS, Sohn HY. 2009. Evaluation of antimicrobial, antithrombin, and antioxidant activity of Dioscorea batatas Decne. Korean J. Microbiol. Biotechnol. 37: 133-139.
16 Kim JM, Kang DH, Kim JH, Na SY, Ju YS. 2007. A study on external and internal morphology in 4 kinds of Uie-Suel radix. Korean J. Herbology 22: 71-79.
17 Kim KS, Kim H. 2011. Morphometrics and distribution of Achyranthes bidentata complex. Korean J. Medicinal Crop Sci. Apr. 28: 86-87.
18 Kim KS, Seung NS, Kim MW, Pyo BS, Baik H. 1997. Micropropagation of Achyranthes japonica through axillary buds culture. Korean J. Plant Tissue Culture 24: 357-360.
19 Kim KS, Shin YW, Kim EI, Kim SM, Lee JE, Yoo DY. 2005. The experimental study on antithrombotic activities of wuslsan. The J. Oriental Obster. Gynecol. 18: 110-126.
20 Kim MS, Jeong JS, Lee HY, Ju YS, Bae GS, Seo SW, et al. 2011. The anti-inflammatory effect of Achyranthes japonica on lipopolysaccharide-induced inflammatory activity in murine macrophages. Korean J. Herbology 26: 51-57.
21 Kim SH, Choi EJ, Kim DH, Lee KY, Lee M, Baek SW, et al. 2008. Stability test of the extracts of Cimicfugae rhizoma, Achyranthis radix, Artemisia capillaris herba, mountain cotex radicis and Arecae semen for toxicity study. Korean J. Pharmacogn. 39: 241-245.
22 Kim SJ, Park JB, Kwon YH, Park KK, Choung SY. 2002. The effect of Achyranthis radix extract on hard tissue regeneration. The J. Appl. Pharmcol. 10: 253-257.
23 Kim YO, Lee SW, Lee SE. 2009. Effect of Achyranthes japonica on carragenan-induced arthritis in rat model. Korean J. Medicinal Crop Sci. 17: 470-474.
24 Choi JY, Lee CH, Jang JB, Lee KS, Lee JM. 2012. Inhibitory effects of Achyranthis radix extract mixed with hydrogel on osteoclast differentiation. The J. Oriental Obster. Gynecol. 25: 1-10.
25 Ahn SM, Ryu HY, Kang DK, Jung IC, Sohn HY. 2009. Antimicrobial and antioxidant activity of the fruit of Prunus avium L. Korean J. Microbiol. Biotechnol. 36: 195-200.
26 Bang SY, Kim JH, Kim HY, Lee YJ, Park SY, Lee SJ, et al. 2012. Achyranthes japonica exhibits anti-inflammatory effect via NF-${\kappa}B$ suppression and HO-1 induction in macrophages. J. Ethnopharmacol. 144: 109-117.   DOI   ScienceOn
27 Cai H, Choi SI, Lee YM, Heo TR. 2002. Antimicrobial effects of herbal medicine extracts on Staphylococcus aureus and Escherichia coli O157:H7. Korean J. Biotechnol. Bioeng. 17: 537-542.
28 Jung SM, Choi SI, Park SM, Heo TR. 2007. Antimicrobial effects of Achyranthes japonica Nakai extract against Clostridium difficile. Korean J. Food Sci. Technol. 39: 564-568.
29 Kang HG, Hong JW, Han HJ, Hwang YY, Jeong JY, Lee KN. 2004. Effects of methanol extract of radix Achyranthis bidentatae on cadmium inhalation toxicity in rat. Korean J. Oriental Physiol. Pathol. 18: 1784-1794.
30 Kim CS, Park YK. 2010. The therapeutic effect of Achyranthis radix on the collagen-induced arthritis in mice. Korean J. Herbology 22: 155-167.
31 Jang GY, Kim HY, Lee SH, Kang Y, Hwang IG, Woo KS, et al. 2012. Effects of heat treatment and extraction method on antioxidant activity of several medicinal plants. J. Korean Soc. Food Sci. Nutr. 41: 914-920.   DOI   ScienceOn