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http://dx.doi.org/10.4014/kjmb.1306.06002

Anti-Oxidative and Anti-Obesity Activities of Tetrapanax papyriferus and Siegesbeckia pubescens Extracts and their Synergistic Anti-Obesity Effects  

Park, Jung Ae (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Jin, Kyong-Suk (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Lee, Ji Young (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Kwon, Hyun Ju (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Kim, Byung Woo (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Publication Information
Microbiology and Biotechnology Letters / v.41, no.3, 2013 , pp. 341-349 More about this Journal
Abstract
In this study, the anti-oxidative and anti-obesity activities of two medicinal herb extracts, Tetrapanax papyriferus (TP) and Siegesbeckia pubescens (SP), were evaluated using DPPH radical scavenging activity assay, lipase enzyme inhibition assay, and the cell culture model system. Both methanol extracts of TP and SP showed DPPH radical scavenging activities dose-dependently, and the $IC_{50}$ of DPPH radical scavenging activities of the two medicinal herbs were 65.23 and 47.79 ${\mu}g/ml$, respectively. Furthermore, both extracts suppressed effectively lipase enzyme activity dose-dependently. Moreover, TP and SP extracts significantly suppressed adipocyte differentiation, lipid accumulation, triglyceride (TG) contents on 3T3-L1 preadipocytes in a dose-dependent manner without cytotoxicity. Their anti-obesity effect was modulated by cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins ${\alpha}$ ($C/EBP{\alpha}$), $C/EBP{\beta}$ and peroxisome proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$) gene and protein expressions. Furthermore, TP and SP possessed a synergistic effect on anti-obesity activity. The identification of the active compounds that confer the anti-obesity activity of TP and SP might be needed.
Keywords
Tetrapanax papyriferus; Siegesbeckia pubescens; anti-oxidative effect; lipase inhibition activity; anti-obesity activity; synergistic effect;
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