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http://dx.doi.org/10.4014/kjmb.1301.01004

Characterization of the Thermophilic Bacterium Geobacillus sp. Strain GWE1 Isolated from a Sterilization Oven  

Correa-Llanten, Daniela (Fundacion Biociencia)
Larrain-Linton, Juanita (Fundacion Biociencia)
Munoz, Patricio A. (Fundacion Biociencia)
Castro, Miguel (Fundacion Biociencia)
Boehmwald, Freddy (Fundacion Biociencia)
Blamey, Jenny M. (Fundacion Biociencia)
Publication Information
Microbiology and Biotechnology Letters / v.41, no.3, 2013 , pp. 278-283 More about this Journal
Abstract
A gram-positive, rod-shaped, spore-forming, motile thermophilic bacterium was isolated from a sterilization oven. The microorganism GWE1, formally named Geobacillus wiegelii identified as a member of the genus Geobacillus. GWE1 grew under aerobic conditions of between $60-80^{\circ}C$ (optimum $670^{\circ}C$), in a pH range of 3.0-8.0 (optimum $pH^{70^{\circ}C}$ 5.8), and between 0 and 2 M NaCl (optimum 0.3 M). The membrane polar lipids were dominated by branched saturated fatty acids, which included as the major constituents; iso-15:0 (13.3%), 16:1(${\omega}7$) (12.8%), 16:0 (28.5%), iso-17:0 (13.5%) and anteiso-17:0 (12.3%). The DNA G+C content was 47.2 mol% (determined by HPLC). The 16S rRNA gene sequence of GWE1 showed a high similarity with Geobacillus caldoxylosilyticus (97%). However, the level of DNA-DNA relatedness was only 58%. These data suggest that GWE1 is probably a novel specie of the genus Geobacillus.
Keywords
Thermophilic bacterium; Geobacillus; sterilization oven;
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