Browse > Article
http://dx.doi.org/10.4014/kjmb.1208.08012

Antibiotic Resistance and Bacterial Biofilm Formation by Staphylococcus aureus Strains Isolated from Various Foods  

Lee, Joo-Young (Food Analysis Center, Korea Food Research Institute)
Wang, Hae-Jin (Food Analysis Center, Korea Food Research Institute)
Shin, Dong-Bin (Food Analysis Center, Korea Food Research Institute)
Cho, Yong-Sun (Food Analysis Center, Korea Food Research Institute)
Publication Information
Microbiology and Biotechnology Letters / v.41, no.1, 2013 , pp. 96-104 More about this Journal
Abstract
Staphylococcus aureus is a major human pathogen that produces a wide array of toxins, leading to a number of adverse symptoms. We examined 275 strains of Staphylococcus aureus isolated from various foods between 2006 and 2008 for antimicrobial susceptibility. At least 259 (94.2%) of the tested strains showed antibiotic resistant properties, and 106 (40.7%) of them showed multiple antibiotic resistance. Eleven of the tested strains were resistant to oxacillin and mec A-positive. Moreover, oxacillin-resistant strains were significantly more likely to be multi-drug resistant (p < 0.01). Of the 275 isolates tested, 24.4% were noted as being positive for slime production and 30.5% were positive for biofilm assay. Antibiotic resistance was not associated with a significantly higher prevalence of biofilm formation. Twenty strains were classified using the DiversiLab system. Most of the strains could be classified into 2 clusters and 4 unique types. All 10 mec A-positive strains (cluster I) were grouped together into the same sub-cluster. Cluster II (6 strains) was not found to be resistant to oxacillin in this study. Although the prevalence of methicillin-resistant S. aureus in food is currently low, the risk of its transmission through the food chain cannot be disregarded.
Keywords
Staphylococcus aureus; antibiotic resistance; biofilm; MRSA;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Chery, K. S., J. I. Pounder, S. R. Page, B. J. Schaecher, and G. L. Woods. 2005. Clinical Evaluation of the DiversiLab microbial typing system using repetitive-sequence-based PCR for characterization of Staphylococcus aureus strains. J. Clin. Microbiol. 43: 1187-1192.   DOI   ScienceOn
2 Chmielewski, R. A. N. and J. F. Frank. 2003. Biofilm formation and control in food processing facilities. Compr. Rev. Food Sci. Food Saf. 2: 22-32.   DOI
3 Cho, K. J., S. Jin, J. H. Cui, T. R. Yoon, and P. Y. Ryu. 2008. Effects of biofilm formation on the antimicrobial susceptibility of Staphylococcus aureus. J. Bacteriol. Virol. 38: 197-205.   DOI   ScienceOn
4 Freeman, D. J., F. R. Falkiner, and C. T. Keane. 1989. New method for detecting slime production by coagulase negative staphylococci. J. Clin. Pathol. 42: 872-874.   DOI
5 Hwang, I. G., H. S. Kwak, and S. H. Yoon. 2010. Methicillinresistant Staphylococcus aureus (MRSA) as a foodborne biological hazard. J. Food Hyg. Safety 50: 26-36.
6 Johannes, K. M., A. H. Matthiab, R. Hologer, and M. Dietrich. 2002. Evaluation of different detection methods of biofilm formation in Staphylococcus aureus. Med. Microbiol. Immunol. 191: 101-106.   DOI
7 KFDA. 2009. Repot of 2009 Estabililshment of a risk profile for methicillin-resistant Staphylococcus aureus (MRSA). Korea Food and Drug Administration, Cheongwon, Korea.
8 KFDA. 2010. Statistical yearbook. Food - borne disease outbreaks, Korea Food and Drug Administration, Cheongwon, Korea.
9 Kim, J. S., H. S. Kim, W. Song, H. C. Cho, K. M Lee, and E. C. Kim. 2004. Antimicrobial ressistat profiles of Staphylococcus aureus isolated in 13 Korea hospitals. Korean J. Lab. Med. 24: 223-229.
10 Kumar, C. G. and S. K. Anand. 1998. Significance of microbial biofilms in food industry. Int. J. Food Microbiol. 42: 9-27.   DOI   ScienceOn
11 Kwon, A.S., G. C. Park,, S. Y. Ryu, D. H. Lim, D. Y. Lim, C. H. Choi, Y. K. Park, and Y. Lim. 2008. Higher biofilm formation in multidrug-resistant clinical isolates of Staphylococcus aureus. Int. J. Antimicrob. Agents 32: 68-72.   DOI   ScienceOn
12 Kwon, Y. I., T. W. Kim, H. Y. Kim, Y. H. Chang, H. S. Kwak, and G. J. Woo. 2007. Monitoring of methicillin resistant Staphylococcus aureus from medical environment in Korea. Korean J. Microbiol. Biotechnol. 35: 158-162.
13 Lee, W. C., T. Sakai, M. J. Lee, M. Hamakawa, S. M. Lee, and I. M. Lee. 1996. An epidemiological study of food poisoning in Korea and Japan. Int. J. Food Microbiol. 29: 141-148 .   DOI   ScienceOn
14 Lim, S. K., H. M. Nam, H. J. Park, H. S. Lee, M. J. Choi, S. C. Jung, J. Y. Lee, Y. C. Kim, S. W. Song, and S. H. Wee. 2010. Prevalence and characterization of methicillin -resistant Staphylococcus aureus in raw meat in Korea. J. Microbiol. Biotechnol. 20: 775-778.
15 Marco, L., B. Cinzia, G. B. Maria, F. Maria, Z. Jessica, and F. Roberta. 2002. Evaluation of the VITEK 2 system for identification and antimicrobial susceptibility testing of medically relevant gram-positive cocci. J. Clin. Microbiol. 40: 1681-1686.   DOI   ScienceOn
16 Park J. I. and N. L. Lee. 1998. Comparison of susceptibility test and meca detection for determination of methicillin resistance in Staphylococcus epidermidis. Korean J. Clin. Pathol. 18: 391-395.
17 Perez-Roth, E., F. C. Martyin, J. Villar, and S. M. Alvarez. 2001. Multiplex PCR for simultaneous identification of Staphylococcus aureus and detection of methicillin and mupirocin resistance. J. Clin. Microbiol. 39: 4037-4041.   DOI   ScienceOn
18 Philip, S. S. and J. W. Costerton. 2001. Antibioric resistance of bacteria in biofilms. J. Lancet. 358: 135-138.   DOI   ScienceOn
19 Rhee, C. H. and G. J. Woo. 2010. Emergence and characterization of foodborne methicillin-resistant Staphylococcus aureus in Korea. J. Food Prot. 73: 2285-2290.
20 Ross, T. L., W. G. Merz, M. Farkosh, and K. C. Carroll. 2005. Comparison of an automated repetitive sequence-based pcr microbial typing system to pulsed-field gel electrophoresis for analysis of outbreaks of methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 43: 5642-5647.   DOI   ScienceOn
21 Shutt, C.K., J. I. Pounder, S. R. Page, B. J. Schaecher, and G. L. Woods. 2005. Clinical evaluation of the diversilab microbial typing system using repetitive-sequence-based PCR for characterization of Staphylococcus aureus strains. J. Clin. Microbiol. 43: 1187-1192.   DOI   ScienceOn
22 Timothy, F. J., M. E. Kellum, S. S. Porter, M. Bell, and W. Schaffner. 2002. An Outbreak of community-acquired foodborne illness caused by methicillin-resistant Staphylococcus aureus. J. Emerg. Infect. Dis. 8: 82-84.   DOI   ScienceOn
23 Vasudevan, P., K. M. N. Manoj, T. Annamalai, and K. S. Venkitanarayanan. 2003. Phenotypic and genotypic characterization of bovine mastitis isolates of Staphylococcus aureus for biofilm formation. Vet. Microbiol. 92: 179-185.   DOI   ScienceOn
24 Wei, H. L. and C. S. Chiou. 2002. Molecular subtyping of Staphylococcus aureus from an outbreak associated with a food handler. Epidemiol. Infect. 128: 15-20.
25 Witta, R., V. Kanhaib, and W. B. Leeuwena. 2009. Comparison of the DiversiLabTM system, pulsed-field gel electrophoresis and multi-locus sequence typing for the characterization of epidemic reference MRSA strains. J. Microbiol. Methods 77: 130-133.   DOI   ScienceOn