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http://dx.doi.org/10.4014/kjmb.1208.08001

Isolation and Characterization of a Novel Agar Degrading Bacterium, Alteromonas macleodii subsp. GNUM08120, from Red Macroalgae  

Chi, Won-Jae (Department of Biological Science, Myongji University)
Lim, Ju-Hyeon (Department of Biological Science, Myongji University)
Park, Da Yeon (Department of Biological Science, Myongji University)
Kim, Mu-Chan (Department of Marine Environmental Engineering, Gyeongsang National University)
Kim, Chang-Joon (Department of Chemical and Biological Engineering, Gyeongsang National University)
Chang, Yong-Keun (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
Hong, Soon-Kwang (Department of Biological Science, Myongji University)
Publication Information
Microbiology and Biotechnology Letters / v.41, no.1, 2013 , pp. 8-16 More about this Journal
Abstract
An agar-hydrolyzing marine bacterium, strain GNUM08120, was isolated from Sargassum fulvellum collected from Yeongil bay of East Sea of Korea. The isolate was Gram-negative, aerobic, motile with single polar flagellum, and grew at 1-10% NaCl, pH 5.0-8.0, and $15-37^{\circ}C$. G+C content and the predominant respiratory quinone were 46.13 mol% and Q-8, respectively. The major cellular fatty acids were Summed feature 3 (24.5%), $C_{16:0}$ (21.7%), and $C_{18:1}{\omega}7c$ (12.5%). Based on 16S rRNA gene sequence similarity and DNA-DNA hybridization analyses, strain GNUM08120 was identified as a novel subspecies of Alteromonas macleodii, designated Alteromonas macleodii subsp. GNUM08120. Production of agarase by strain GNUM08120 was likely repressed by the effect of carbon catabolite repression caused by glucose. The crude agarase prepared from 12-h culture broth of strain GNUM08120 exhibited an optimum pH and temperature for agarase activity at 7.0 and $40^{\circ}C$, respectively. The crude enzyme produced (neo)agarobiose, (neo)agarotetraose, and (neo)agarohexaose as the hydrolyzed product of agarose.
Keywords
Agarase; Alteromonas macleodii; red macroalgae; phylogenetic tree;
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1 Angell, S., C. G. Lewis, M. J. Buttner, and M. J. Bibb. 1994. Glucose repression in Streptomyces coelicolor A3(2): a likely regulatory role for glucose kinase. Mol. Gen. Genet. 244: 135-143.
2 Ausubel, S. F., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1989. Current Protocols in Molecular Biology. New York: Wiley.
3 Bajpai, P. 1997. Microbial xylanolytic enzyme system: properties and applications. Adv. Appl. Microbiol. 43: 141-194.   DOI
4 Baumann, L., P. Baumann, M. Mandel, and R. D. Allen. 1972. Taxomy of aerobic marine eubacteria. J. Bacteriol. 110: 402-429.
5 Beg, Q. K., M. Kapoor, L. Mahajan, and G. S. Hoondal. 2001. Microbial xylanases and their industrial applications: a review. Appl. Micriobiol. Biotechnol. 56: 326-338.   DOI
6 Chi, W. J., D. Y. Park, S. C. Jeong, Y. K. Chang, and S. K. Hong. 2011. Isolation and characterization of starch-hydrolyzing Pseudoalteromonas sp. A-3 from the coastal sea water of Daecheon, Republic of Korea. Korean J. Microbiol. Biotech. 39: 317-323.
7 Chi, W. J., Y. K. Chang, and S. K. Hong. 2012. Agar degradation by microorganisms and agar-degrading enzymes. Appl. Microbiol. Biotechnol. 94: 917-930.   DOI   ScienceOn
8 Chiu, H. H., W. Y. Shieh, S. Y. Lin, C. M. Tseng, P. W. Chiang, and I. Wagner-Dobler. 2007. Alteromonas tagae sp. nov. and Alteromonas simiduii sp. nov., mercury-resistant bacteria isolated from a Taiwanense estuary. Int. J. Syst. Evol. Microbiol. 57: 1209-1121.   DOI   ScienceOn
9 Chun, J. S., J. H. Lee, Y. Y. Jung, M. J. Kim, S. I. Kim, B. K. Kim, and Y. W. Lim. 2007. Extaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57: 2259-2261.   DOI   ScienceOn
10 Englard, S. and S. Seifter. 1990. Precipitation techniques. Methods Enzymol. 182: 285-300.   DOI
11 Felsenstein, J. 1985. Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39: 783-791.   DOI   ScienceOn
12 Ivanova, E. P., J. P. Bowman, A. M. Lysenko, N. V. Zhukova, N. M. Gorshkova, A. F. Sergeev, and V. V. Mikhailov. 2005. Alteromonas addita sp. nov. Int. J. Syst. Evol. Microbiol. 55: 1065-1068.   DOI   ScienceOn
13 Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge Univesity Press, UK.
14 Kirimura K, N. Masuda, Y. Iwasaki, H. Nakagawa, R. Kobayashi, and S. Usai. 1999. Purification and characterization of a novel β-agarase from an alkalophilic bacterium, Altermonas sp. E-1. J. Biosci. Bioeng. 87: 436-441.   DOI   ScienceOn
15 Kobayashi, R., M. Takisada, T. Suzuki, K. Kirimura, and S. Usami. 1997. Neoagarobiose as a novel moisturizer with whitening effect. Biosci. Biotechnol. Biochem. 61: 162-163.   DOI   ScienceOn
16 Komagata, K. and K. Suzuki. 1987. Lipid and cell-wall analysis in bacterial systematic. Methods Microbiol. 19: 161-207.
17 Kwakman, J. H. and P. W. Postma. 1994. Glucose kinase has a regulatory role in carbon catabolite repression in Streptomyces coelicolor. J. Bacteriol. 176: 2694-2698.
18 Leon, O., L. Quintana, G. Peruzzzo, and J. C. Slebe. 1992. Purification and properties of an extracellular agarase from Alteromonas sp. strain C-1. Appl. Environ. Microbiol. 58: 4060-4063.
19 Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
20 Martinez-Checa, F., W. Bejar, I. Llamas, A. del Moral, and E. Quesda. 2005. Alteromonas hispanica sp. nov., a polyunsaturated- fatty-acid-producing, halophilic bacterium isolated from Fuente de Piedra, southern Spain. Int. J. Syst. Evol. Microbiol. 55: 2385-2390.   DOI   ScienceOn
21 McCandless, E. 1981. Polysaccharides of the seaweeds. In: Lobban, C, and M. Wynne. (eds) The biology of seaweeds. University of California Press, Berkeley, pp. 559-588.
22 Mesbah, M., U. Premachandran, and W. B. Whitman. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol. 39: 159-167.   DOI
23 Miller, L. and T. Berger. 1985. Bacterial identification by gas chromatography of whole cell fatty acid. Hewlett-Packard Application note. pp. 228-241.
24 Miyazono, K., N. Tabei, S. Morita, Y. Ohnishi, S. Horinouchi, and M. Tanokura. 2012. Substrate recognition mechanism and substrate-dependent conformational changes of an ROK family glucokinase from Streptomyces griseus. J. Bacteriol. 194: 607-616.   DOI   ScienceOn
25 Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428.   DOI
26 Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.
27 Sasser, M. 1997. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI Inc.
28 Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positionspecific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680.   DOI   ScienceOn
29 Van Trappen, S., T. L. Tan, J. Yang, J. Mergaert, and J. Swings. 2004. Alteromonas stellipolaris sp. nov., a novel, budding, prosthecate bacterium from Antarctic seas, and emended description of the genus Alteromonas. Int. J. Syst. Evol. Microbiol. 54: 1157-1163.   DOI   ScienceOn
30 Temuuji, U., W. J. Chi, S. Y. Lee, Y. K. Chang, and S. K. Hong. 2011. Overexpression and biochemical characterization of DagA from Streptomyces coelicolor A3(2): an endo-type $\beta$- agarase producing neoagarotetraose and neoagarohexaose. Appl. Microbiol. Biotechnol. 92: 749-759.   DOI   ScienceOn
31 Wang, J., H. Mou, X. Jiang, and H. Guan. 2006. Characterization of a novel beta-agarase from marine Altermonas sp. SY37-12 and its degrading products. Appl. Microbiol. Biotechnol. 71: 833-839.   DOI   ScienceOn
32 Yoon, J. H., I. G. Kim, K. H. Kang, T. K. Oh, and Y. H. Park. 2003. Alteromonas marina sp. nov., isolated from sea water of the East Sea in Korea. Int. J. Syst. Evol. Microbiol. 53: 125-130.   DOI   ScienceOn
33 Yoon, J. H., S. H. Yeo, T. K. Oh, and Y. H. Park. 2004. Alteromonas litorea sp. nov., a slightly halophilic bacterium isolated from an intertidal sediment of the Yellow Sea in Korea. Int. J. Syst. Evol. Microbiol. 54: 1197-1201.   DOI   ScienceOn