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http://dx.doi.org/10.4014/kjmb.1209.09002

Design of Short Indolicidin Analogs with Enhanced Prokaryotic Selectivity  

Shin, Song Yub (Department of Cellular & Molecular Medicine, School of Medicine, Chosun University)
Publication Information
Microbiology and Biotechnology Letters / v.40, no.4, 2012 , pp. 409-413 More about this Journal
Abstract
Indolicidin (ID) is a 13-residue Trp-rich antimicrobial peptide (AMP) isolated from bovine neutrophils. In addition to having a high antimicrobial potency, it is also toxic to mammalian cells. To develop novel ID-derived AMPs with shorter lengths and enhanced prokaryotic selectivities (meaning potent antimicrobial activity against bacterial cells without toxicity against mammalian cells) over the parental ID, several ID analogs were designed and synthesized. Finally, 10-residue ID analogs (SI, SI-PA, SI-WF and SI-WL) with much higher prokaryotic selectivity than the parental ID were developed. Our results suggest that the hydrophobic and aromatic amino acids at the central position of the analog SI with the highest prokaryotic selectivity are important for potent antimicrobial activity, but two Pro residues do not affect antimicrobial activity. The order of prokaryotic selectivity for ID and its designed analogs was SI > SI-PA > SI-WF > SI-WL > ID > SI-WA. Taken together, our designed short ID analogs could be developed as therapeutic agents for treating bacterial infections.
Keywords
antimicrobial peptide; indolicidin; short analogs; prokaryotic selectivity;
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