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http://dx.doi.org/10.4014/kjmb.1207.07005

Effect of Inhibited Rab11-FIP3 Expression on Membrane Trafficking of TRPV1 in Dorsal Root Ganglion of Rat  

Kim, Miran (Bioneer Corporation)
Lee, Soon-Youl (Department of Biotechnology, Hankyong National University)
Publication Information
Microbiology and Biotechnology Letters / v.40, no.3, 2012 , pp. 278-281 More about this Journal
Abstract
Not much is known about the membrane trafficking of TRPV1, a key player in pain transduction. Rab11-FIP3, which plays a role in various intracellular transportation pathways, has been reported to interact with TRPV1. In this study, in order to examine the role of Rab11-FIP3 in the membrane trafficking of TRPV1, Rab11-FIP3 expression in dorsal root ganglion (DRG) was inhibited using a siRNA technique. Transportation of TRPV1 to membranes was found to decrease when Rab11-FIP3 expression was inhibited, consistent with the results obtained with TRPV1-transfected HEK cells. Taken together, these results indicate that Rab11-FIP3 plays a role in the membrane trafficking of TRPV1.
Keywords
TRPV1; Rab11-FIP3; small interfering RNA (siRNA); membrane trafficking; dorsal root ganglion (DRG);
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