Browse > Article
http://dx.doi.org/10.4014/kjmb.1206.06003

Optimization of Conditions for the Production of Alginate-degrading Crude Enzyme from Vibrio crassostreae PKA 1002  

SunWoo, Chan (Department of Food Science & Technology/Institute, Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Department of Food Science & Technology/Institute, Pukyong National University)
Kim, Dong-Hyun (Department of Food Science & Technology/Institute, Pukyong National University)
Jung, Seul-A (Department of Food Science & Technology/Institute, Pukyong National University)
Kim, Hyun-Jee (Department of Food Science & Technology/Institute, Pukyong National University)
Jeong, Da-Hyun (Department of Food Science & Technology/Institute, Pukyong National University)
Jung, Hee-Ye (Department of Food Science & Technology/Institute, Pukyong National University)
Lim, Sung-Mee (Department of Food Science & Technology Tongmyong University)
Hong, Yong-Ki (Department of Biotechnology, Pukyong National University)
Ahn, Dong-Hyun (Department of Food Science & Technology/Institute, Pukyong National University)
Publication Information
Microbiology and Biotechnology Letters / v.40, no.3, 2012 , pp. 243-249 More about this Journal
Abstract
This study was conducted to screen an alginate-degrading microorganism and to investigate the characteristics of the alginate-degrading activity of its crude enzyme. A marine bacterium which produces extracellular alginate-degrading enzymes was isolated from the brown alga Sargassum thunbergii. 16S rRNA sequence analysis and physiological profiling resulted in the bacterium's identification as a Vibrio crassostreae strain, named Vibrio crassostreae PKA 1002. Its optimal culture conditions for growth were pH 9, 2% NaCl, $30^{\circ}C$ and a 24 hr incubation time. The optimal conditions for the alginate degrading ability of the crude enzyme produced by V. crassostreae PKA 1002 were pH 9, $30^{\circ}C$, a 48 hr incubation time and 8% alginic acid. The alginate degrading crude enzyme produced 3.035 g of reducing sugar per liter in 4% (w/v) alginate over 1 hr.
Keywords
Alginate-degrading crude enzyme; Vibrio crassostreae PKA 1002;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Yumoto, I., H. Iwata, T. Sawabe, K. Ueno, N. Ichise, H. Matsuyama, H. Okuyama, and K. Kawasaki. 1999. Chracterization of a facultatively psychoropholic bacterium, Vibrio rumoiensis sp. nov., that exhibits high catalase activity. Appl. Environ. Microb. 65: 67-72.
2 Anzai, H., H. Asada, A. Koshiba, S. Yoshida, H. Kobayashi, N. Uchida and E. Nishide. 1991. Distribution of polysaccharide digestive enzymes in a marine gastropod Dolabella auricularia. Nippon Suisan Gakk. 57: 2077-2081.   DOI
3 Choi, J. H. and D. W. Kim. 1997. Effect of alginic acid-added functional drink (HAETOMINI) brown algae (Undaria pinnatifuda) on obesity and biological activity of SD rats. Kor. J. Life Sci. 7: 361-370.
4 Choi, J. H., C. H. Rhim, J. Y. Kim, J. S. Yang, J. S. Choi, and D. S. Byun. 1986. Basic studies on the development of diet for the treatment of obesity: the inhibitory effect of alginic acid as a dietary fiber on obesity. J. Kor. Fish. Soc. 19: 303-311.
5 Cho, M., B.Y. Kim, and J. H. Rhim. 2003. Degradation of alginate solution by using ${\gamma}$-irradiation and organic acid. Kor. J. Food Sci. Technol. 35: 67-71.
6 Eppley, R. W. and R. Lasker. 1959. Alginase in the sea urchin Strongylocentrotus purpuratus. Science 129: 214-215.   DOI
7 Fisher, F. G. and H. Dorfel. 1955. The polyuronic acids of brown algae. Physiol. Chem. 302: 186-203.   DOI
8 Gacesa, P. 1988. Alginates. Carbohydr. Polym. 8: 161-182.   DOI   ScienceOn
9 Huamao Y., J. Song, L. Xuegang, L. Ning, and D. Jicui. 2006. Immunomodulation and antitumor activity of ${\kappa}$-carrageenan oligosaccharides. Cancer Lett. 243: 228-234.   DOI   ScienceOn
10 Joo, D. S., Y. S. Choi, and S. Y. Cho. 2003. Preparation of the depolymerized alginates by physical treatment processing with organic acids. J. Kor. Fish. Soc. 36: 1-5.
11 Joo, D. S., J. S. Lee, J. J. Park, S. Y. Cho, C. B. Ahn, and E. H. Lee. 1995. Purification and charaterization of the intracellular alginase from Vibrio sp. AL-145. Kor. J. Appl. Microbiol. Biotechnol. 23: 432-438.
12 Kang, S. I., Y. M. Kim, Y. B. Jang, D. J. Lim, and J. Y. Kong. 2004. The optimal culture condition for the collagenolytic protease production from Vibrio vulnificus CYK279H. Kor. J. Biotechnol. Bioeng. 19: 295-300.
13 Kim, J. H., Y. H. Kim, S. K. Kim, B. W. Kim, and S. W. Nam. 2011. Properites and industrial applications of seaweed polysaccharides degrading enzymes from the marine microorganisms. Kor. J. Microbiol. Biotechnol. 39: 189-199.
14 Kim, I. H. and T. J. Nam. 2005. Effect of polymannuronate on cholesterol contents of liver tissue and feces in rats. J. Kor. Soc. Food Sci. Nutr. 34: 1553-1558.   DOI
15 Kim, J. Y., K. N. Kim, J. G. Kim, S. C. Kim, W. J. Lee, and C. G. Hyun. 2009. In vitro antimicrobial and antioxidant activities of chitosan oligosaccharides. J. Appl. Biol. Chem. 52: 84-87.   DOI
16 Kim, O. J., D. G. Lee, S. M. Lee, S. J. Lee, H. J. Do, H. J. Park, A. Kim, J. H. Lee, and J. M. Ha. 2010. Isolation and characteristics of alginate-degrading Methylobacterium sp. HJM27. Kor. J. Microbiol. Biotechnol. 38: 144-150.
17 Kim, H. K., J. C. Lee, N. H. Kang, S. H. Kim, J. G. Kim, and K. C. Shung. 2007. Purification and charaterization of the extracellular alginate lyase from Streptomyces sp. MET 0515. J. Life Sci. 17: 625-633.   DOI
18 Kobayashi, T., K. Uchimura, M. Miyazaki, Y. Nogi, and K. Horikoshi. 2009. A new high-alkaline alginate lyase from a deep-sea bacterium Agarivorans sp.. Extrmophiles 13: 121-129.   DOI   ScienceOn
19 Kennedy, L., K. Mcdowell, and I. W. Sutherland. 1992. Alginases from Azotobacter species. J. Gen. Microbiol. 138: 2465-2471.   DOI
20 Liyan, L., J. Xialu, G. Huashi, W. Peng, and G. Hong. 2011. Three alginate lyases from marine bacterium Pseudomonas fluorescens. HZJ216: purification and characterization. Appl. Biochem. Biotech. 164: 305-317.   DOI   ScienceOn
21 Lee, J. H., M. J. Bae, Y. C. Kim, and S. W. Nam. 2009. Identification and charaterization of alginate lyase producing Pseudomonas sp. N7141-6. Kor. J. Microbiol. Biotechnol. 37: 350-354.
22 Li, J. W., S. Dong, J. Song, C. B. Li, X. L. Shen, B. B. Xie, and Y. Z. Zhang. 2011. Purification and characterization of bifunctional alginate lyase from Pseudoalteromonas sp. SM0524. Marine Drugs 9: 109-123.   DOI
23 Mulligan, M. S., J. B. Lowe, R. D. Larsen, J. Paulson and Z. L. Zheng, S. Defress, K. Maemura, and M. Fukuda, P. A. Ward. 1993. Protective effects of sialyated oligosaccharides in immun complex induced acute lung injury. J. Exp. Med. 178: 623-631.   DOI   ScienceOn
24 Muramatsu, T., S. Hirose, and M. Katayose. 1977. Isolation and properties of alginate lyase from the mid gut gland of wreath shell Turbo cornutus. Agric. Biol. Chem. 41: 1939-1946.   DOI
25 Nelson, A. 1944. A photometric adaptation of the somogyi method for the determination of glucose. J. Biol. Chem. 153: 375-380.
26 Richard, A. S. and R. E. Levein. 1976. Viscometric assay of bacterial alginase. Appl. Eviro. Microbiol. 31: 896-899.
27 Smidsrod, O., A. Haug, and B. Larsen. 1963. Degrading of alginate in the prosence of reducing compounds. Acta. Chem Scand. 17: 2628-2637.
28 Takeshita, S, N. Sato, M. Igarashi, and T. Muramatsu. 1993. A highly denaturant durable alginate lyase from a marine bacterium: purification and proterties. Biosci. Biotech. Bioch. 57: 1125-1128.   DOI
29 Stevens, R. A. and R. E. Levin. 1977. Purification and charateristics of an alginase from Alginovibrio aquatilis. Appl. Environ. Microb. 3: 1156-1161.
30 Suntherland, I. W. and G. A. Kenn. 1981. Alginaese from Beneckea pelagia and Pseudomonas spp. J. Appl. Biochem. 3: 48-57.
31 Thiang, Y. W., L. A. Preston, and N. L. Schiller. 2000. Alginate lyase: review of major sources and enzyme characteristics structure-function analysis, biological roles and applications Annu. Rev. Microbial. 54: 289-340.   DOI   ScienceOn
32 Uo, M. H., D. S. Joo, and S. Y. Joo. 2006. Screening and cultivation characteristics of alginate degrading bacteria. J. Kor. Soc. Food Sci. Nutr. 35: 109-114.   DOI
33 Wataru, H., O. Masako, H. Tomohiro, M. Keiko, and M. Kousaku. 1998. Sphingomonas sp. Al lyase active on both poly-${\beta}$-D-mannuronate and heteropolymeric regions alginate. J. Ferment. Bioeng. 86: 236-238.   DOI   ScienceOn
34 Xiating, F., H. Lin, and S. M. Kim. 2008. Optimization of culturing condition and medium composition for the production of alginate lyase by a marine Vibrio sp. YKW-34. J. Ocean Univ. China 7: 97-102.   DOI   ScienceOn
35 Yoshiko, I., A. Ryoko, I. Kenichi, O. Tatsuya, F. Hisataka, H. Shinziro, and M. Tsuyoshi. 2001. Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain No. 272 and its action on saturated oligomeric substrates. BioSci. Biotech. Bioch. 65: 133-142.   DOI   ScienceOn