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http://dx.doi.org/10.4014/kjmb.1202.02002

Properties of ${\beta}$-Galactosidase from Bacillus licheniformis Isolated from Cheongkookjang  

Yoon, Ki-Hong (Department of Food Science & Biotechnology, Woosong University)
Publication Information
Microbiology and Biotechnology Letters / v.40, no.1, 2012 , pp. 17-22 More about this Journal
Abstract
A bacterial strain was isolated from homemade Cheongkookjang as a producer of the ${\beta}$-galactosidase, capable of hydrolyzing lactose to liberate galactose and glucose residues. The isolate YB-1105 has been identified as Bacillus licheniformis on the basis of its 16S rDNA sequence, morphology and biochemical properties. ${\beta}$-Galactosidase activity was detected in both the culture supernatant and the cell extract of B. licheniformis YB-1105. The enzymes of both fractions demonstrated maximum activity for hydrolysis of para-nitrophenyl-${\beta}$-D-galactopyranoside (pNP-${\beta}Gal$) under identical reaction conditions of pH 6.5 and $50^{\circ}C$. However, ${\beta}$-galactosidase activity from the culture filtrate was affected more than that from the cell free extract at acidic pHs and high temperatures. The hydrolyzing activity of both ${\beta}$-galactosidases for pNP-${\beta}Gal$ was dramatically decreased by the addition of low concentrations of galactose, but was only marginally decreased by high concentrations of glucose or mannose.
Keywords
Bacillus licheniformis; identification; ${\beta}$-galactosidase; property;
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