Browse > Article

Antimicrobial and Hemolytic Activity of Oriental Medicinal Herbs  

Ryu, Hee-Young (Dept. of Food and Nutrition, Andong National University)
Ahn, Seon-Mi (Dept. of Food and Nutrition, Andong National University)
Shin, Yong-Kyu (Research Institute of Biotechnology, Bion Co., LTD.)
Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
Publication Information
Microbiology and Biotechnology Letters / v.38, no.2, 2010 , pp. 190-197 More about this Journal
Abstract
To develop the safe and natural antimicrobial agents, the 68 ethanol extracts from the 61 different kinds of oriental herbal medicine were prepared and their antimicrobial activities were evaluated. The herbal medicine used were from China (46 kinds), South Korea (14 kinds), North Korea (5 kinds) and Vietnam (3 kinds), respectively, and the root (27 species) was popular part in this study. The average water content and extraction ratio for ethanol were 7.10% and 6.75%, respectively. Determination of antimicrobial activity by disc-diffusion assay at 0.5 mg/disc concentration showed that the extract of Angelica tenuissima Nakai (china), Illicium verum, Junci medulla, Rhus javanica L., Salvia miltiorrhiza Bunge and Syzygium aromaticum has strong antimicrobial activities against different food spoilage and pathogenic bacteria and fungi. Determination of MIC and MBC/MFC further showed that the extract of Syzygium aromaticum has MIC of 1.25 mg/mL and MBC/MFC of 1.25~5.00 mg/mL against Listeria monocytogenes, Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus aureus, Salmonella typhimurium, Proteus vulgaris, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Saccharomyces cerevisiae. And, the extract of Junci medulla, Rhus javanica L. and Salvia miltiorrhiza Bunge showed strong antibacterial activities with MIC of 0.08~0.63 mg/mL and MBC/MFC of 0.08~2.50 mg/mL against the tested bacteria except E. coli and P. aeruginosa. In a while, the results of hemolytic activity of 68 different herbal extracts against human red blood cells showed that the extract of Angelica tenuissima Nakai has hemolytic activity at 0.5 mg/mL concentration. Therefore, Illicium verum, Junci medulla, Rhus javanica L., Salvia miltiorrhiza Bunge and Syzygium aromaticum were finally selected for natural antimicrobial resources. Further research on active substances and the mode of action of the selected herbal medicine is necessary.
Keywords
Antimicrobial activity; MIC/MBC/MFC; hemolytic activity; Oriental medicinal herbs;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
Times Cited By SCOPUS : 3
연도 인용수 순위
1 Han, W. S. 2004. Isolation of antimicrobial compounds from Salvia miltiorrhiza Bunge. Kor. J. Med. Crop Sci. 12: 179-182.
2 Oh, D. H., S. S. Ham, B. K. Park, C. Ahn, and J. Y. Yu. 1998. Antimicrobial activities of natural medicinal herbs on the food spoilage or foodborn disease microorganisms. Kor. J. Food Sci. Technol. 30: 957-963.
3 Do, J. R., K. J. Kim, J. H. Jo, Y. M. Kim, B. S. Kim, H. K. Kim, S. D. Lim, and S. W. Lee. 2005. Antimicrobial, antihypertensive and anticancer activities of medicinal herbs. Kor. J. Food Sci. Technol. 37: 206-213.
4 Grayer, R. J. and J. B. Harbone. 1994. A survey of antifungal compounds from higher plants. Phytochemistry 37: 19-24.   DOI   ScienceOn
5 Ryu, H. Y., K. H. Bae, E. J. Kum, S. J. Park, B. H. Lee, and H. Y. Sohn. 2007. Evaluation of the antimicrobial, antioxidant and antithrombosis activity of natural spices for fresh-cut yam. J. Life Sci. 17: 652-657.   DOI
6 Lee, S. E., C. G. Park, M. S. Cha, J. K. Kim, N. S. Seong, K. H. Bang, and J. K. Bang. 2002. Antimicrobial activity of essential oils from Mentha arvensis L. and Agastache rugosa on Escherichia coli and Salmonella typhimurium. Kor. J. Med. Crop Sci. 10: 206-211.
7 Lee, C. B. 2003. Illustrated flora of Korea. Hwang Moon Sa, Seoul.
8 Ahn, S. M., H. Y. Ryu, D. K. Kang, I. C. Jung, and H. Y. Sohn. 2009. Antimicrobial and antioxidant activity of ther fruit of Prunus avium L. Kor. J. Microbiol. Biotechnol. 37: 371-376.
9 Board, R. G. 1969. The microbiology of the hens egg. In advances in Applied Microbiology. Vol. 2. D. Perlman, Ed. Academic Press, New York.
10 Ryu, H. Y., I. S. Kwun, S. J. Park, B. H. Lee, and H. Y. Sohn. 2007. Inhibition of browning in yam fresh-cut and control of yam-putrefactive bacterium using acetic acid or maleic Acid. Kor. J. Microbiol. Biotechnol. 35: 135-141.
11 Phengpaichit, S., N. Pujenjob, V. Rukachaisirikul and M. Ongsakul. 2005. Antimicrobial activities of the crude methanol extract of Acorus calamus Linn. Songklanakarin J. Sci. Technol. 27: 517-523.
12 Beuchat, L. R. and D. A. Golden. 1989. Antimicrobial occurring naturally in foods. Food Technol. 43: 131-141.
13 Lewis, R. J. 1989. Their regulatory status their use by the food industry. In Food additives handbook, Robert W. D. (Ed), Nostrand Reinhold, New York, p. 3-27.
14 Woo, S. S., Y. K. Park, C. H. Choi, K. S. Hahm, and D. G. Lee. 2007. Mode of antibacterial action of a signal peptide, $Pep^{27}$ from Streptococcus pneumoniae. Biochem. Biophys. Res. Comm. 363: 806-810.   DOI   ScienceOn
15 Scott, V. N. 1988. Safety considerations for new generation refrigerated foods. Dairy Food Environ. Sanitation 8: 5-8.
16 Zaika, L. L. 1988. Spices and herbs: their antimicrobial activity and its determination. J. Food Safety. 9: 97-101.
17 Cai, H., S. I. Choi, Y. M. Lee, and T. R. Heo. 2002. Antimicrobial effects of herbal medicinal extracts on Staphylococcus aureus and Escherichia coli O157:H7. Kor. J. Biotechnol. Bioeng. 17: 537-542.
18 Han, S. H., N. R. Y. Woo, S. D. Lee, and M. H. Kang. 2006. Antioxidative and antibacterial activities of endemic plants extracts in Korea. Kor. J. Med. Crop Sci. 14: 49-55.
19 Ahn, D. J., Y. S. Kwak, M. J. Kim, J. C. Lee, C. S. Shin, and K. T. Jeong. 2000. Screening of herbal plant extracts showing antimicrobial activity against some food spoilage and pathogenic microorganisms. Kor. J. Med. Crop Sci. 8: 109-116.