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Screening of $\alpha$-Amylase and $\alpha$-Glucosidase Inhibitor from Nepalese Plant Extracts  

Kim, Mi-Sun (Dept. of Food and Nutrition, Andong National University)
Ahn, Seon-Mi (Dept. of Food and Nutrition, Andong National University)
Jung, In-Chang (Dept. of Food and Nutrition, Andong National University)
Kwon, Gi-Seok (The School of Bioresource Science, Andong National University)
Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
Publication Information
Microbiology and Biotechnology Letters / v.38, no.2, 2010 , pp. 183-189 More about this Journal
Abstract
In the course of screening for anti-acidosis and anti-diabetes agent from natural products, the inhibitory activities of Nepales plant extracts against microbial $\alpha$-amylase and $\alpha$-glucosidase were evaluated. Among the 100 different kinds of ethanol extracts, Cedrus deodara (Roxb.) G. Don and Myrica nagi Thunb showed strong inhibition activities against $\alpha$-amylase. The $IC_{50}$ values of C. deodara (Roxb.) G. Don, M. nagi Thunb and acarose, a commercial available anti-diabetes agent, were 44.5, 47.5 and $50.5\;{\mu}g/mL$, respectively. Considering the crude extract of C. deodara (Roxb.) G. Don, and M. nagi Thunb, these extracts have strong potentials as anti-acidosis or anti-diabates agent. In a while, Cleistocalyx operculatus (Roxb.) extract showed a good inhibition activity to $\alpha$-amylase and $\alpha$-glucosidase, even it was recently reported. The selected three extracts did not show any hemolysis activity against human red blood cell up to 1 mg/mL, and the inhibition activities were maintained by heat or acid treatment. Moreover, treatment of HCl (0.01N) for 1 h into C. operculatus (Roxb.) and M. nagi Thunb increased the inhibition activity from 50% to 70%. Our results suggest that C. deodara (Roxb.) G. Don, M. nagi Thunb, and C. operculatus (Roxb.) are potential as anti-acidosis and anti-diabetes agents.
Keywords
Cedrus deodara; Cleistocalyx operculatus; Myrica nagi; nepalese plant extract; $\alpha$-amylase inhibitor; $\alpha$-glucosidase inhibitor;
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