Browse > Article

Detection of Novel Polyketide Synthase Genes in Sorangium cellulosum Isolated in Korea  

Youn, Jin-Kwon (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Kim, Do-Hee (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Lee, Han-Bit (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Lee, Kye-Won (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Cho, Kyung-Yun (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Publication Information
Microbiology and Biotechnology Letters / v.38, no.2, 2010 , pp. 136-143 More about this Journal
Abstract
DNA fragments encoding the ketosynthase (KS) domain of polyketide synthase (PKS) genes were amplified using polymerase chain reaction (PCR) from 9 strains of Sorangium cellulosum isolated in Korea, cloned into a plasmid vector and sequenced. A total of 83 cloned DNA fragments were analyzed, and similar fragments were excluded, leaving 43 independent DNA fragments encoding the KS domains. The predicted amino acid sequences of 32 fragments were 70%-100% identical to the amino acid sequences of already known PKS genes, while the remaining 11 fragments were $\leq$67% or less identical to the known sequences, suggesting that these genes are novel PKS genes.
Keywords
Myxobacteria; polyketide synthase; PKS; Sorangium cellulosum;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Julien, B., Z. Q. Tian, R. Reid, and C. D. Reeves. 2006. Analysis of the ambruticin and jerangolid gene clusters of Sorangium cellulosum reveals unusual mechanisms of polyketide biosynthesis. Chem. Biol. 13: 1277-1286.   DOI   ScienceOn
2 Weissman, K. J. and R. Müller. 2009. A brief tour of myxobacterial secondary metabolism. Bioorg. Med. Chem. 17: 2121-2136.   DOI   ScienceOn
3 Schneiker, S., O. Perlova, O. Kaiser, K. Gerth, A. Alici, M. O. Altmeyer, D. Bartels, T. Bekel, S. Beyer, E. Bode, H. B. Bode, C. J. Bolten, J. V. Choudhuri, S. Doss, Y. A. Elnakady, B. Frank, L. Gaigalat, A. Goesmann, C. Groeger, F. Gross, L. Jelsbak, L. Jelsbak, J. Kalinowski, C. Kegler, T. Knauber, S. Konietzny, M. Kopp, L. Krause, D. Krug, B. Linke, T. Mahmud, R. Martinez-Arias, A. C. McHardy, M. Merai, F. Meyer, S. Mormann, J. Munoz-Dorado, J. Perez, S. Pradella, S. Rachid, G. Raddatz, F. Rosenau, C. Ruckert, F. Sasse, M. Scharfe, S. C. Schuster, G. Suen, A. Treuner-Lange, G. J. Velicer, F.J. Vorholter, K. J. Weissman, R. D. Welch, S. C. Wenzel, D. E. Whitworth, S. Wilhelm, C. Wittmann, H. Blocker, A. Puhler, and R. Muller. 2007. Complete genome sequence of the myxobacterium Sorangium cellulosum. Nat. Biotechnol. 25: 1281-1289.   DOI   ScienceOn
4 Sambrook, J., E. F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
5 Li, Z. F., J. Y. Zhao, Z. J. Xia, J. Shi, H. Liu, Z. H. Wu, W. Hu, W. F. Liu, and Y. Z. Li. 2007. Evolutionary diversity of ketoacyl synthases in cellulolytic myxobacterium Sorangium. Syst. Appl. Microbiol. 30: 189-196.   DOI   ScienceOn
6 Hyun, H., J. Chung, H. Lee, J. Youn, C. Lee, D. Kim, and K. Cho. 2009. Isolation of cellulose-degrading myxobacteria Sorangium cellulosum. Kor. J. Microbiol. 45: 48-53.
7 Gerth, K., S. Pradella, O. Perlova, S. Beyer, and R. Muller. 2003. Myxobacteria: proficient producers of novel natural products with various biological activities-past and future biotechnological aspects with the focus on the genus Sorangium. J. Biotech. 106: 233-253.   DOI
8 Bode, H. B. and R. Müller. 2006. Analysis of myxobacterial secondary metabolism goes molecular. J. Ind. Microbiol. Biotechnol. 33: 577-588.   DOI   ScienceOn
9 Gerth, K., O. Perlova, and R. Muller. 2008. Sorangium cellulosum. pp. 329-348. In Whitworth, D. E. (ed.), Myxobacteria: multicellularity and differentiation. ASM Press, Washington, D.C.
10 Staunton, J. and K. J. Weissman. 2001. Polyketide biosynthesis: a millennium review. Nat. Prod. Rep. 18: 380-416.   DOI   ScienceOn
11 Staunton, J. and B. Wilkinson. 2001. Combinatorial biosynthesis of polyketides and nonribosomal peptides. Curr. Opin. Chem. Biol. 5: 159-164.   DOI   ScienceOn
12 Reichenbach, H. and M. Dworkin. 1992. The myxobacteria. pp. 3416-3487. In Balows, A., H.G. Trüper, M. Dworkin, W. Harder, and K.-H. Schleifer (ed.), The Prokaryotes, 2nd ed., vol. IV, Springer Verlag, NY.
13 Ronning, C. M. and W. C. Nierman. 2008. The genomes of Myxococcus xanthus and Stigmatella aurantiaca, pp. 285-298. In Whitworth, D. E. (ed.), Myxobacteria: multicellularity and differentiation. ASM Press, Washington, D.C.
14 Reichenbach, H. 2005. Myxococcales. pp. 1059-1144. In Brenner, D.J., N.R. Krieg, J.T. Staley, and G.M. Garrity (ed.), Bergey's Manual of Systematic Bacteriology, 2nd ed. Bergey's Manual Trust, East Lansing, MI.
15 Hyun, H., J. Chung, J. Kim, J. S. Lee, B. -M. Kwon, K. -H. Son, and K. Cho. 2008. Isolation of Sorangium cellulosum carrying epothilone gene clusters. J. Microbiol. Biotechnol. 18: 1416-1422.
16 Schirmer, A., R. Gadkari, C. D. Reeves, F. Ibrahim, E. F. DeLong, and C. R. Hutchinson. 2005. Metagenomic analysis reveals diverse polyketide synthase gene clusters in microorganisms associated with the marine sponge Discodermia dissoluta. Appl. Environ. Microbiol. 71: 4840-4849.   DOI   ScienceOn
17 Frank, B., J. Knauber, H. Steinmetz, M. Scharfe, H. Blocker, S. Beyer, and R. Müller. 2007. Spiroketal polyketide formation in Sorangium: identification and analysis of the biosynthetic gene cluster for the highly cytotoxic spirangienes. Chem. Biol. 14: 221-233.   DOI   ScienceOn
18 Johnson, M., I. Zaretskaya, Y. Raytselis, Y. Merezhuk, S. McGinnis, and T. L. Madden. 2008. NCBI BLAST: a better web interface. Nucleic Acids Res. 36: W5-W9.   DOI   ScienceOn
19 Larkin, M. A., G. Blackshields, N. P. Brown, R. Chenna, P. A. McGettigan, H. McWilliam, F. Valentin, I. M. Wallace, A. Wilm, R. Lopez, J. D. Thompson, T. J. Gibson, and D. G. Higgins. 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947-2948.   DOI   ScienceOn
20 Komaki, H., R. Fudou, T. Iizuka, D. Nakajima, K. Okazaki, D. Shibata, M. Ojika, and S. Harayama. 2008. PCR detection of type I polyketide synthase genes in myxobacteria. Appl. Environ. Microbiol. 74: 5571-5574.   DOI   ScienceOn
21 Finking, R. and M. A. Marahiel. 2004. Biosynthesis of nonribosomal peptides. Annu. Rev. Microbiol. 58: 453-488.   DOI   ScienceOn
22 Cane, D. E., C. T. Walsh, and C. Khosla. 1998. Harnessing the biosynthetic code: combinations, permutations, and mutations. Science 282: 63-68.   DOI
23 Kim, Y. S., W. C. Bae, and S. J. Back. 2003. Bioactive substances from myxobacteria. Kor. J. Microbiol. Biotechnol. 31: 1-12.
24 Julien, B., S. Shah, R. Ziermann, R. Goldman, L. Katz, and C. Khosla. 2000. Isolation and characterization of the epothilone biosynthetic gene cluster from Sorangium cellulosum. Gene 16: 153-160.
25 Molnar, I., T. Schupp, M. Ono, R. Zirkle, M. Milnamow, B. Nowak-Thompson, N. Engel, C. Toupet, A. Stratmann, D. D. Cyr, J. Gorlach, J. M. Mayo, A. Hu, S. Goff, J. Schmid, and J. M. Ligon. 2000. The biosynthetic gene cluster for the microtubule-stabilizing agents epothilones A and B from Sorangium cellulosum So ce90. Chem. Biol. 7: 97-109.   DOI   ScienceOn