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Large-Circular Single-stranded Sense and Antisense DNA for Identification of Cancer-Related Genes  

Bae, Yun-Ui (Department of Physiology, Yeungnam University School of Medicine)
Moon, Ik-Jae (WelGENE Inc.)
Seu, Young-Bae (School of Life Sciences & Biotechnology, Kyungpook National University)
Doh, Kyung-Oh (Department of Physiology, Yeungnam University School of Medicine)
Publication Information
Microbiology and Biotechnology Letters / v.38, no.1, 2010 , pp. 70-76 More about this Journal
Abstract
The single-stranded large circular (LC)-sense DNA were utilized as probes for DNA chip experiments. The microarray experiment using LC-sense DNA probes found differentially expressed genes in A549 cells as compared to WI38VA13 cells, and microarray data were well-correlated with data acquired from quantitative real-time RT-PCR. A 5K LC-sense DNA microarray was prepared, and the repeated experiments and dye swap test showed consistent expression patterns. Subsequent functional analysis using LC-antisense library of overexpressed genes identified several genes involved in A549 cell growth. These experiments demonstrated proper feature of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense microarray and antisense libraries for an effective functional validation of genes.
Keywords
Microarray; expression profiling; microarray; antisense; lung cancer;
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