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Development of pSJE6c, an Expression Vector for Kimchi Lactic Acid Bacteria, and Heterologous Gene Expression Using the Vector  

Lee, Kang-Wook (Division of Applied Life Science (BK21 program), Graduate School)
Park, Ji-Yeong (Division of Applied Life Science (BK21 program), Graduate School)
Lee, Ji-Yeon (Division of Applied Life Science (BK21 program), Graduate School)
Lee, Hwang-A (Division of Applied Life Science (BK21 program), Graduate School)
Baek, Chang-Un (Division of Applied Life Science (BK21 program), Graduate School)
Jo, Hyeon-Deok (Division of Applied Life Science (BK21 program), Graduate School)
Kim, Joo-Yeon (Division of Applied Life Science (BK21 program), Graduate School)
Kwon, Gun-Hee (Institute of Agriculture & Life Science, Gyeongsang National University)
Chun, Ji_Yeon (Department of Food Science and Technology, Sunchon National University)
Kim, Jeong-Hwan (Division of Applied Life Science (BK21 program), Graduate School)
Publication Information
Microbiology and Biotechnology Letters / v.37, no.4, 2009 , pp. 389-398 More about this Journal
Abstract
Development of expression vectors is important for the basic and applied researches on kimchi LAB (lactic acid bacteria). An expression vector, pSJE6c was constructed by inserting P6C promoter sequence from Lactococcus lactis into pSJE, a shuttle vector for E. coli and Leuconostoc species. To test the efficiency of pSJE6c, aga ($\alpha$-galactosidase) and lacZ ($\beta$-galactosidase) genes were expressed in Lactobacillus brevis 2.14. Compared to the pSJE, expression levels of both genes were increased, indicating P6C promoter was better than indigenous promoters. Enzyme activities of L. brevis cells harboring pSJE6caga (pSJE6c with aga) or pSJE6Z (pSJE6c with lacZ) were 1.5-2 fold higher than those with pSJEaga (pSJE with aga) or pSJEZ (pSJE with lacZ). More RNA transcripts were detected in cells harboring pSJE6c based recombinant plasmid. The results indicated that heterologous gene expressions in kimchi LAB could be improved significantly by use of efficient expression vectors.
Keywords
Lactobacillus brevis; $\alpha$-galactosidase; $\beta$-galactosidase; expreion vectors; promoters;
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