Browse > Article

Identification and Characterization of Alginate Lyase Producing Pseudomonas sp. N7151-6  

Lee, Jae-Hyung (Department of Biomaterial Control, Dong-Eui University)
Bae, Min-Ji (Department of Biotechnology & Bioengineering, Dong-Eui University)
Kim, Yang-Choon (Gijang Local Products Co., Ltd.)
Nam, Soo-Wan (Department of Biomaterial Control, Dong-Eui University)
Publication Information
Microbiology and Biotechnology Letters / v.37, no.4, 2009 , pp. 350-354 More about this Journal
Abstract
A Gram-negative, alginate lyase-producing bacterium was isolated from the Haeundae Coast, Korea. The isolated strain N7151-6 produced alginate lyase. The optimal temperature and pH for growth were found to be $30^{\circ}C$ and pH 8.0, respectively. This strain can be grown at the NaCl concentration of 0-7% (w/v). Analysis of 16S rDNA sequence and physiological profiling indicated that the strain N7151-6 belonged to Pseudomonas sp. The enzyme alginate lyase produced by Pseudomonas sp. N7151-6 was partially purified by ultrafiltration (MWCO= 30 kDa). The optimum pH and temperature for the activity of the purified enzyme were found to be 7.0 and $30^{\circ}C$, respectively. The enzyme was stable at the pH range of 5.0-9.0 and temperature range of $23-30^{\circ}C$. The total activity of alginate lyase produced was reached about 110 unit/L.
Keywords
Alginate lyase; Pseudomonas sp.; 16S rDNA; physiological reaction profiling;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 4
연도 인용수 순위
1 Penman, A. and G. R. Sanderson. 1972. A method for the determination of uronic acid sequence in alginates. Carbohydrate Res. 25: 273-282   DOI   ScienceOn
2 Sawabe, T., H. Takahashi, Y. Ezura, and P. Gacesa. 2001. Cloning, sequence analysis and expression of Pseudoalteromonas elyakoii IAM 14594 gene (alyPEEC) encoding the extracellular alginate lyase. Carbohydr. Res. 335: 11-21   DOI   ScienceOn
3 Stevens, R. A. and R. E. Levin. 1977. Purification and characteristics of an alginate from Alginovibrio aquatilis. Appl. Environ. Microbiol. 3: 1156-1161
4 Tamaoka, J. and K. Komagata. 1984. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol. Lett. 25: 125-128   DOI   ScienceOn
5 Dunne, W. M. and F. L. Buckmire. 1985. Partial purification and characterization of a polymannuronic acid depolymerase produced by a mucoid strain of Pseudomonas aeroguinosa isolated from a patient with cystic fibrosis. Appl. Environ. Microbiol. 50: 562-567   PUBMED   ScienceOn
6 Hirst, E. L. and D. A. Rees. 1965. The structure of alginic acid. Part V. Isolation and unambiguous characterization of some hydrolysis products of the methylated polysaccharide. J. Chem. Society. 7: 1182-1187   DOI   ScienceOn
7 Nakagawa A., T. Ozaki, K. Chubachi, T. Hosoyama, T. Okubo, S. Iyobe, and T. Suzuki. 1998. An effective method for isolating alginate lyase-producing Bacillus sp. ATB-1015 strain and purification and characterization of the lyase. J. Appl. Microbiol. 84: 328-335   DOI   ScienceOn
8 Pecina, A., A. Pascual, and A. Paneque. 1999. Cloning and expression of the algL gene, encoding the Azotobacter chroococcum alginate lyase: purification and characterization of the enzyme. J. Bacteriol. 181: 1409-1414   PUBMED   ScienceOn
9 Haug. A., B. Larsen, and O. Smidsrod. 1966. A study of constitution of alginic acid by partial acid hydrolysis. Acta. Chemica. Scandinabica. 20: 183-190   DOI
10 Franklin, M. J., C. E. Chitnis, P. Gacesa, A. Sonesson, D. C. White, and D. E. Ohman. 2007. Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase. J. Bacteriol., 186: 4759-4773   DOI   ScienceOn
11 Hansen, J. B. and L. K. Nakamura. 1985. Distribution of alginate lyase activity among strains of Bacillus circulans. Appl. Environ. Microbiol. 49: 1019-1021   PUBMED   ScienceOn
12 Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428   DOI
13 Paxman, J. R., J. C. Richardson, P. W. Dettmar, and B. M. Corfe. Alginate reduces the increased uptake of cholesterol and glucose in overweight male subjects: a pilot study. 2008. Nutr. Res. 28: 501-505   DOI   ScienceOn
14 Joo, D. S., S. Y. Cho, and E. H. Lee. 1993. Isolation of alginate-degrading bacteria and production of alginatedegrading activities by the bacteria. Kor. J. Appl. Microbiol. Biotechnol. 21: 207-213
15 Skerman, V. B. D. 1967. A Guide to the Identification of the Genera of Bacteria, 2nd edn. Batimore: Williams & Wilkins
16 Guven, K. C., Y. Ozsoy, and O. N. Ulutin. 1991. Anticoagulant, fibrinotic and antiaggregant activity of carrageenans and alginic acid. Biotanica. Marina. 34:429-435   DOI
17 Linker, A. and L. R. Evans. 1984. Isolation and characterization of an alginase from mucoid strains of Pseudomonas aeroguinosa. J. Bacteriol. 159: 958-964   PUBMED
18 Davidson, I. W., I. W. Sutherland, and C. J. Lawson. 1976. Purification and properties of an alginate lyase from a marine bacterium. Biochem. J. 159: 707-713   PUBMED
19 Haug, A., B. Larsen, and O. Smidsrod. 1967. Studies on the sequence of uronic acid residues in alginic acid. Acta. Chemica. Scandinabica. 21: 691-704   DOI
20 Lanyi, B. 1987. Classical and rapid identification methods for medically important bacteria. pp. 1-67. In Colwell, R.R and R. Grigorova (ed.), Methods in Microbiology, vol. 19, Academic Press Ltd., London
21 Iwamoto, Y., R. Araki, K. Iriyam, T. Oda, H. Fukuda, S. Hayashida, and T. Muramatsu. 2001. Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain no. 272 and its action on saturated oligomeric substrates. Biosci. Biotechnol. Biochem. 65: 133-142   DOI   ScienceOn
22 Jeong, H. J., S. A. Lee, P. D. Moon, H. J. Na, R. K. Park, J. Y. Um, H. M. Kim, and S. H. Hong. Alginic acid has antianaphylactic effects and inhibits inflammatory cytokine expression via suppression of nuclear factor-kappaB activation. 2006. Clin. Exp. Allergy. 36: 785-794   DOI   ScienceOn
23 Joo, D. S., J. S. Lee, J. J. Park, S. Y. Cho, C. B. Ahn, and E. H. Lee. 1995. Purification and characterization of the intracellular alginase from Vibrio sp. AL-145. Kor. J. Appl. Microbiol. Biotechnol. 23: 432-438
24 Lee, J. H. and E. Y. Lee. 2003. Isolation of alginatedegrading marine bacteria and characterization of alginase. J. Life Science. 13: 718-722   DOI
25 Anzai, H., N. Uchida, and E. Nishida. 1990. Determination of D-mannuronic to L-guluronic acids ratio in acid hydrolysis under improved conditions. Nippon Suisan Gakkaishi. 56: 73-81   DOI