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Isolation and Medium Development of the Actinomycetes, Streptomyces griseofuscus CNU-A91231, Inhibiting Phytopathogenic Fungi  

Choi, Seung-Hyun (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University)
Son, Min-Jung (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University)
Kim, Sung-Han (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University)
Choi, Suk-Yul (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University)
Lee, Yoon-Hui (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University)
Choi, Jae-Eul (Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University)
An, Gil-Hwan (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
Microbiology and Biotechnology Letters / v.37, no.4, 2009 , pp. 322-332 More about this Journal
Abstract
Five hundred strains of actinomycetes were screened for inhibitory activity against the phytopathogenic fungi; Alternaria alternata, Botryotinia fuckeliana, Colletotrichum acutatum, Colletotrichum gleosporioides, Corticium sasaki, Cylindrocarpon destructans, Fusarium oxysporium, Magnaporthe grisea, Phytophthora infestans, Phytium ultimum, and Thanatephorus cucumeris. The strain CNU-A91231 (Korea Agricultural Culture Collection #20938) showed a strong activity against the phytopathogenic fungi and it was identified as Streptomyces griseofuscus based on the sequence of 16s rDNA. Practical and simple media for the strain S. griseofuscus CNU-A91231 was developed at the conditions ($28^{\circ}C$ and pH 6 with aeration) for efficient bacterial growth. Alanine, glutamine, proline and ammonium ion were good nitrogen sources for the bacterium. Addition of the major salts including Na, Cl, Ca, P, K, and Mg into molasses did not increase the growth of S. griseofuscus. Addition of fertilizers containing amino acids significantly enhanced growth of the bacterium. The optimal medium was formulated as molasses + 1% of glutamate fermentation waste powder. All the conditions and components used in this study did not affect the antifungal activity of S. griseofuscus. The bacterium and the medium in this study can be used as a bio-antifungal agent for plant farming.
Keywords
Actinomycetes; medium; phytopathogenic fungi; Streptomyces griseofuscus;
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1 Agrios, G. N. 1997. Plant pathology. 5th ed. Elsevier Academic Press, Burlington, MA, U.S.A
2 An, G. H., B. G. Jang, and M. H. Cho. 2001. Cultivation of the carotenoid-hyperproducing mutant 2A2N of the red yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) with molasses. J. Biosci. Bioeng. 92: 121-125   DOI   ScienceOn
3 Demain, A. l. and N. A. Solomon. 1986. Manual of industrial microbiology and biotechnology, pp. 216-263. American society for Microbiology, Washington D.C., NY, U.S.A
4 Idris, H. A., Labuschagne N., and Korsten L. 2008. Suppression of Pythium ultimum root rot of sorghum by rhizobacterial isolates from Ethiopia and South Africa. Biol. Control. 45: 72-84   DOI   ScienceOn
5 Justen, A. F., D. Yohalem, A. Bay, and M. Nicolaisen. 2003. Genetic diversity in potato field populations of Thanatephorus cucumeris AG-3, revealed by ITS polymorphism and RAPD markers. Mycol. Res. 107: 1323-1331   DOI   ScienceOn
6 Mounier, J., M. C. Rea, P. M. O'Connor, G. F. Fitzgerald, and T. M. Cogan. 2007. Growth Characteristics of Brevibacterium, Corynebacterium, Microbacterium, and Staphylococcus spp. Isolated from Surface-Ripened Cheese. Appl. Environ Microbiol. 73: 7732-7739   DOI   ScienceOn
7 Sonawane, A. M., B. Singh, and K. H. Rohm. 2006. The AauR-AauS Two-Component System Regulates Uptake and Metabolism of Acidic Amino Acids in Pseudomonas putida. Appl. Environ. Microbiol. 72: 6569-6577   DOI   ScienceOn
8 Wang, Y., B. Yihong, D. Shen, W. Feng, T. Yu, Z. Jia, and X. D. Zheng. 2008. Biocontrol of Alternaria alternate on cherry tomato fruit by use of marine yeast Rhodosporidium paludigenum Fell and Tallman. Int. J. Food. Microbiol. doi:10.1016/j.ijfoodmicro.2008.02.002   DOI   ScienceOn
9 Kin, S. L. 2006. Discovery of novel metabolites from marine actinomycetes. Curr. Opin. Microbiol. 8: 245-251   DOI   ScienceOn
10 Gabriele, S. M., M. Rosselli, and B. Imthurn. 2008. Comparison of two procedures for routine IUD exchange in woman with positive Pap smears for actinomyces-like organisms. Contraception. 77: 177-180   DOI   ScienceOn
11 Leroux, P., F. Chapeland, D. Desbrosses, and M. Gredt. 1999. Patterns of cross-resistance to fungicides in Botryyotinia fuckeliana(Botrytis cinerea) isolates from French vineyards. Crop Prot. 18: 687-697   DOI   ScienceOn
12 Tongen, A., A. Goriely, and M. Tabor. 2006. Biomechanical model for appressorial design in Magnaporthe grisea. J. Theor. Biol. 240: 1-8   DOI   ScienceOn
13 Park, C. S. 1984. Effects of soil solarization for control of cucumber wilt. Kor. J. Plant Prot. 23: 22-27
14 Ripoche, A., G. Jacqua, F. Bussiere, S. Guyader, and J. Sierra. 2008. Survival of Colletotrichum gloeosporioides (casual agent of yam anthracnose) on yam residues decomposing in soil. Appl. Soil Ecol. 38: 270-278   DOI   ScienceOn
15 Reuveni, M. 2006. Inhibition of germination and growth of Alternaria alternate and mouldy-core development in red delicious apple fruit by bromuconazole and sygnum. Crop Prot. 25: 253-258   DOI   ScienceOn
16 Sharma, A., V. Vivekanand, and R. P. Singh. 2008. Solidstate fermentation for gluconic acid production from sugarcane molasses by Aspergillus niger ARNU-4 employing tea waste as the novel solid support. Bioreso. Technol. 99: 3444-3450   DOI   ScienceOn
17 Bradford, M. M. 1976. A rapid and sensitive methods for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254   DOI   PUBMED   ScienceOn
18 Hur, Y. A., S. S. Choi, Y. K. Chang, S. K. Hong, and E. S. Kim. 2007. Solid medium pH-dependent antifungal activity of Streptomyces sp. producing an immunosuppressant tautomycetin. Kor. J. Microbiol. Biotechnol. 35: 26-29
19 Kernaghan, G., R. D. Reeleder, and S. M. T. Hoke. 2007. Quantification of Cylindrocarpon destructans f. sp. panacis in soils by real-time PCR. Plant Pathol. 56: 508-516   DOI   ScienceOn
20 AOAC. 1995. Official Method of Analysis. 16th ed., The Assn. of Ofiic. Anal. Chem., Washington D.C., U.S.A
21 Skottup, P., M. Nicolaisen, and A. F. Justesen. 2007. Rapid determination of Phytophthora infestans sporangia using a surface plasmon resonance immunosensor. J. Microbiol. Methods 68: 507-515   DOI   ScienceOn