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Isolation of Chondromyces crocatus in Pure Culture  

Lee, Cha-Yul (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Hyun, Hye-Sook (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Kim, Do-Hee (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Cho, Kung-Yun (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Publication Information
Microbiology and Biotechnology Letters / v.37, no.4, 2009 , pp. 316-321 More about this Journal
Abstract
We have isolated Chondromyces crocatus KYC2823 in pure culture and five other strains in mixed culture with companion bacteria from Korean soil samples. The strain KYC2823, which was isolated from the soil sample collected in Cheongdo-gun, Gyeongsangbuk-do, showed typical characteristics of C. crocatus, including the shape of fruiting bodies and production of a peculiar odor. In addition, the 16S rDNA sequence was 99.8% identical to that of the strain Cm c5, the proposed neotype strain of C. crocatus. Cloning and sequence analysis of the polyketide biosynthetic genes from KYC2823 by performing PCR have revealed that this strain has biosynthetic gene clusters for ajudazols (inhibitors of electron transport systems) and chondramides (substances affecting the function of the actin cytoskeleton), and biosynthetic genes for other polyketide compounds that have not been cloned yet.
Keywords
Myxobacteria; chondromyces crocatus; chondromide; ajudazol;
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