Browse > Article

Isolation of Myxobacteria Carrying Soraphen Biosynthetic Gene Clusters  

Lee, Cha-Yul (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Hyun, Hye-Sook (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Cho, Kung-Yun (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
Publication Information
Microbiology and Biotechnology Letters / v.37, no.1, 2009 , pp. 10-16 More about this Journal
Abstract
Polymerase chain reactin (PCR) could be a simple way to screen new microbial strains producing useful secondary metabolites if their biosynthetic genes are known and candidate strains to be screened are available. In this study, we have screened two myxobacterial strains, KYC3047 and KYC3076, carrying genes appeared to be biosynthetic genes of soraphen A, a potent antifungal substance, out of 50 cellulose degrading myxobacteria using PCR. The two strains were identified as Sorangium cellulosum based on morphological, physiological, and molecular biological characteristics. Both of the strains produced substances having strong antifungal activities as expected against Candida albicans, a causative agent of candidiasis, and Colletotrichum acutatum, a causative agent of anthracnose on pepper.
Keywords
Myxobacteria; Sorangium cellulosum; soraphen;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Hill, A. M. and B. L. Thompson. 2003. NoveL soraphens from precursor directed biosynthesis. Chem. Commun. 21: 1360-1361
2 Park, S. H. and H. W. Lee. 2008. Synthetic studies on soraphen A: synthesis of the C1-C9 hemiketal segment. Bull. Korean Chem. Soc. 29: 1445-1446   DOI   ScienceOn
3 Schupp, T. C. Toupet, B. Cluzel, S. Neff, S. Hill, J. J. Beck, J. M. Ligon. 1995. A Sorangium cellulosum (myxobacterium) gene cluster for the biosynthesis of the macrolide antibiotic soraphen A:cloning, characterization, and homology to poiyketide synthase genes from actinomycetes. J. Bacteriol. 177: 3673-3679   PUBMED   ScienceOn
4 Sprer, C., H. Reichenbach, and E. Stackebrandt. 1999. The correlation between morphological and phylogenetic classification of myxobacteria. Int. J. Syst. Bacteriol. 3: 1255-1262   DOI
5 Chung, J., C. Lee, S.-C. Yun, and K. Cho. 2008. Screening of myxobacteria inhibiting the growth of Collectotrichum acutatum causing anthracnose on pepper. Kor. J. Microbiol. Biotechnol. 36: 21-27 (in Korean with English abstract)
6 Reichenbach, H. 2005. Myxococcales. pp. 1059-1144. ln Brenner, D. J., N. R. Krieg, J. T. Staley, and G. M. Garrity(eds.), Bergey's Manual of Systematic Bacteriology, 2nd ed. Bergey's Manual Trust, East Lansing, MI
7 Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680   DOI   ScienceOn
8 Gerth, K., S. Pradella, O. Perlova, S. Beyer, and R. Mller. 2003. Myxobacteria: proficient producers of novel natural products with various biologica1 activities - past and future biotechnological aspects with the focus on the genus Sorangium. J. Biotechnol. 106: 233-253   DOI   ScienceOn
9 Zirkle, R., J. M. Ligon, and I. Molnr I. 2004. Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces Itvidans. Microbiology 150: 2761-2774   DOI   ScienceOn
10 Gerth, K., N. Bedorf, H. Irschik, G. Hfle, and H. Reichenbach. 1994. The soraphens: a farni1y of novel anti fungal compounds from Sorangium cellulosum (Myxobacteria). J. Antibiot. 47: 23-31   PUBMED   ScienceOn
11 Shen, Y., S. L. Volrath, S. C. Weatherly, T. D. Elich, and L. Tong. 2004. A mechanism for the potent inhibition of eukaryotic acetyl-coenzyme A carboxylase by soraphen A, a macrocyclic polyketide natural product. Mol. Cell. 16: 881-891   DOI   ScienceOn
12 Beckers, A., S. Organe, L. Timmermans, K. Scheys, A. Peeters, K. Brusselmans, G. Verhoeven, and J. V. Swinnen. 2007. Chemical inhibition of acetyl-CoA carboxylase induces growth arrest and cytotoxicity selectively in cancer cells. Cancer Res. 67: 8180-8187   DOI   ScienceOn
13 Reichenbach, H. and M. Dworkin. 1992. The myxobacteria. pp. 3416-3487. ln Balows, A., H. G Trper, M. Dworkin, W. Harder, and K. H. Schleifer (eds.), The Prokaryotes, 2nd ed. vol. IV,Springer Verlag, New York
14 Rainey, F. A., Rainey, W. N., Kroppenstedt, R. M., and Stackerbrandt, E. 1996. The Genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. lnt. J. Syst. Bacteriol. 46: 1088-1092   DOI   ScienceOn
15 Lee, H. W., I.-Y. Choi Lee, Y.-S. Kim, and S.-U. Park. 2002 An efficient synthesis of C3-C9 segment of soraphen A. Bull. Korean Chem. Soc. 23: 1197-1198   과학기술학회마을   ScienceOn
16 Kim, Y. S., W. C. Bae, and S. J. Back. 2003. Bioactive substances from myxobacteria. Kor. J. Microbiol. Biotechnol. 31: 1-12 (in Korean with English abstract)   DOI   ScienceOn
17 Ligon J, S. Hill, J. Beck, R. Zirkle, I. Mo1nr, J. Zawodny, S. Money, and T. Schupp. 2002. Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. Gene 285: 257-267   DOI   PUBMED   ScienceOn
18 Vahlensieck, H. F., L. Pridzun, H. Reichenbach, and A. Hinnen. 1994. Identification of the yeast ACCl gene product (acetyl-CoA carboxylase) as the target of the polyketide fungicide soraphen A. Curr. Genet. 25: 95-100   DOI   ScienceOn
19 Zhang, H., B. Tweel, J. Li, and L. Tong, L. 2004. Crystal structure of the carboxyltransferase domain of acetylcoenzyme A carboxylase in complex with CP-640186. Structure 12: 1683-1691   DOI   ScienceOn
20 Park, S. H., H. W. Lee, S.-U. Park. 2004. Synthesis of C3-C9 suLfonyL derivative of soraphen A. Bull. Korean Chem. Soc. 25: 1613-1614   DOI   ScienceOn
21 Zhang, H., Z. Yang, Y. Shen, and L. Tong. 2003, Crystal structure of the carboxyltransferase domain of acetylcoenzyme A carboxylase. Science 299: 2064-2067   DOI   PUBMED   ScienceOn