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Selection of the Auxin and ACC Deaminase Producing Plant Growth Promoting Rhizobacteria from the Coastal Sand Dune Plants  

Lim, Jong-Hui (Department of Applied Microbiology, Yeungnam University)
Kim, Jong-Guk (Department of Microbiology, Kyungpook National University)
Kim, Sang-Dal (Department of Applied Microbiology, Yeungnam University)
Publication Information
Microbiology and Biotechnology Letters / v.36, no.4, 2008 , pp. 268-275 More about this Journal
Abstract
In order to develop the multi-functional rhizobacteria that can exert positive effect on the growth of plants growing in the coastal sand dune located along East Coast of Korea, rhizospheral bacteria of 11 different plants from this area were isolated 1,330 rhizobacteria. Among these, 23 strains were able to produce auxin and had spectrum of antagonism toward various phytopathogenic microbes. To know the mechanism of this antifungal activity, these 23 strains were subjected to further analyses; 19 strains of these produced siderophore as determined by color reaction on CAS-blue plate, 4 strains produced antifungal cellulase as judged by color change on CMC-Congo red plate, 17 strains were able to utilized insoluble phosphate salts, also determined by clear zone formation on PVK medium. Identification of the strain was assigned to all 23 strains by l6s rDNA sequence analysed, and all were identified to be in the genus of Bacillus and Pseudomonas. One strain of these, denoted Pseudomonas fluorescens IB4-14, showed ACC deaminase activity which is known to be involved in the resistance of environmental stress such as salt and drought. Also, P. fluorescens IB4-l4 showed the germination stimulation and roots growth promoting activity on the in vivo assay of Lysimachia mauritiana Lam. (spoonleaf yellow loosestrife).
Keywords
sand dune plant; auxin; ACC deaminase; PGPR;
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