Browse > Article

Molecular Cloning of a Putative Gene Encoding Phospholipase B (plbA) from Aspergillus nidulans  

Hong, Sa-Hyun (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korean National Institute of Health)
Cho, Eun-Min (Metabolome Analysis Team, Seoul Center, Korea Basic Science Institute)
Song, Seung-Eun (Metabolome Analysis Team, Seoul Center, Korea Basic Science Institute)
Eom, Chi-Yong (Metabolome Analysis Team, Seoul Center, Korea Basic Science Institute)
Publication Information
Microbiology and Biotechnology Letters / v.36, no.3, 2008 , pp. 189-194 More about this Journal
Abstract
The phospholipase B (PLB) families are enzymes sharing phospholipase (PL), lysophospholipase (LPL) and lysophospholipase-transacylase (LPTA) activities. In this study, we report the putative gene encoding phospholipase B (plbA) containing lipase motifs was cloned for the first time from the filamentous fungus, Aspergillus nidulans. plbA was isolated from A. nidulans genomic DNA library using a PCR-amplified probe, which is designed on the basis of sequence information derived from the conserved lipase regions of various PLBs. The deduced product of plbA is of 626 amino acids. From the assigned sequence, PlbA showed 72% identity with Penicillium notatum PLB but have low similarity with phospholipase A of other organisms.
Keywords
Aspergillus nidulans; fungi; Phospholipase B; PLB; plbA;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Fyrst, H., B. Oskouian, F. A. Kuypers, and J. D. Saba. 1999. The PLB2 gene of Saccharomyces cerevisiae confers resistance to lysophosphatidylcholine and encodes a phospholipase B/lysophospholipase. Biochem. 38: 5864-5871   DOI   ScienceOn
2 Lee, K. S., J. L. Patton, M. Fido, L. K. Hines, S. D. Kohlwein, F. Paltauf, S. A. Henry, and D. E. Levin. 1994. The Saccharomyces cerevisiae PLB1 gene encodes a protein required for lysophospholipase and phospholipase B activity. J Biol. Chem. 269: 19725-19730
3 Tamura, M., T. Ajayi, L. R. Allmond, K. Moriyama, J. P. Wiener-Kronish, and T. Sawa. 2004. Lysophospholipase A activity of Pseudomonas aeruginosa type III secretory toxin ExoU. Biochem. Biophys. Res. Commun. 316: 323-331   DOI   ScienceOn
4 Rowlands, R. T. and G. Turner. 1973. Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans. Mol. Gen. Genet. 126: 201-16   DOI
5 Cox, G. M., H. C. McDade, S. C. Chen, S. C. Tucker, M. Gottfredsson, L. C. Wright, T. C. Sorrell, S. D. Leidich, A. Casadevall, M. A. Ghannoum, and J. R. Perfect. 2001. Extracellular phospholipase activity is a virulence factor for Cryptococcus neoformans. Mol. Microbiol. 39: 166-175   DOI   ScienceOn
6 Ghannoum, M. A. 2000. Potential role of phospholipases in virulence and fungal pathogenesis. Clin. Microbiol. Rev. 13: 122-143   DOI
7 Johnson, D. R., R. S. Bhatnagar, L. J. Knoll, and J. I. Gordon. 1994. Genetic and biochemical studies of protein N-myristoylation. Annu. Rev. Biochem. 63: 869-914   DOI   ScienceOn
8 Wang, A. and E. A. Dennis. 1999. Mammalian lysophospholipases. Biochim. Biophys. Acta. 1439: 1-16   DOI   PUBMED   ScienceOn
9 Takemori, H., F. N. Zolotaryov, L. Ting, T. Urbain, T. Komatsubara, O. Hatano, M. Okamoto, and H. Tojo. 1998. Identification of functional domains of rat intestinal phospholipase B/lipase. Its cDNA cloning, expression, and tissue distribution. J. Biol. Chem. 273: 2222-2231   DOI   ScienceOn
10 Leidich, S. D., A. S. Ibrahim, Y. Fu, A. Koul, C. Jessup, J. Vitullo, W. Fonzi, F. Mirbod, S. Nakashima, Y. Nozawa, and M. A. Ghannoum. 1998. Cloning and disruption of CaPLB1, a phospholipase B gene involved in the pathogenicity of Candida albicans. J. Biol. Chem. 273: 26078-26086   DOI
11 Saito, K., J. Sugatani, and T. Okumura. 1991. Phospholipase B from Penicillium notatum. Methods Enzymol. 197: 446-456   DOI
12 Chen, S. C., L. C. Wright, J. C. Golding, and T. C. Sorrell. 2000. Purification and characterization of secretory phospholipase B, lysophospholipase and lysophospholipase/transacylase from a virulent strain of the pathogenic fungus Cryptococcus neoformans. Biochem. J. 347: 431-439   DOI
13 Ibrahim, A. S., F. Mirbod, S. G. Filler, Y. Banno, G. T. Cole, Y. Kitajima, J. E. Edwards Jr, Y. Nozawa, and M. A. Ghannoum 1995. Evidence implicating phospholipase as a virulence factor of Candida albicans. Infect. Immun. 63: 1993-1998   PUBMED
14 Reynolds, L., L. Hughes, A. Louis, R. Kramer, and E. Dennis 1993. Metal ion and salt effects on the phospholipase $A_{2}$, lysophospholipase, and transacylase activities of human cytosolic phospholipase$A_{2}$. Biochim. Biophys. Acta. 1167: 272-280   DOI   PUBMED   ScienceOn
15 Masuda, N., N. Kitamura, and K. Saito. 1991. Primary structure of protein moiety of Penicillium notatum phospholipase B deduced from the cDNA. Eur. J. Biochem. 202: 783-787   DOI   ScienceOn
16 Loo, R. W., K. Conde-Frieboes, L. J. Reynolds, and E. A. Dennis. 1997. Activation, inhibition, and regiospecificity of the lysophospholipase activity of the 85-kDa group IV cytosolic phospholipase $A_{2}$. J. Biol. Chem. 272: 19214-19219   DOI   ScienceOn
17 Shen, D. K., A. D. Noodeh, A. Kazemi, R. Grillot, G. Robson, and J. F. Brugere. 2004. Characterisation and expression of phospholipases B from the opportunistic fungus Aspergillus fumigatus. FEMS Microbiol. Lett. 239: 87-93   DOI   ScienceOn
18 Schultz, J., F. Milpetz, P. Bork, and C. P. Ponting. 1998. SMART, a simple modular architecture research tool: identification of signaling domains. Proc. Natl. Acad. Sci. USA. 95: 5857-64
19 Merkel, O., M. Fido, J. A. Mayr, H. Pruger, F. Raab, G. Zandonella, S. D. Kohlwein, and F. Paltauf. 1999. Characterization and function in vivo of two novel phospholipases B/lysophospholipases from Saccharomyces cerevisiae. J. Biol. Chem. 274: 28121-28127   DOI
20 Hanel, A. and M. Gelb. 1995. Multiple enzymatic activities of the human cytosolic 85 kDa phospholipase $A_{2}$: hydrolytic reactions and acyl transfer to glycerol. Biochem. 34: 7807-7818   DOI   ScienceOn