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The Effects of Genistein on the Proliferation and Type I pN Collagen Synthesis in Aged Normal Human Fibroblasts  

Yang, Eun-Soon (Department of Bioengineering, graduate School at KonKuk University)
Hong, Ran-Hi (Department of Preventive Medicine, College of Medicine, ChungAng University)
Kang, Sang-Mo (Department of Bioengineering, graduate School at KonKuk University)
Publication Information
Microbiology and Biotechnology Letters / v.35, no.4, 2007 , pp. 316-324 More about this Journal
Abstract
We studied the effects of genistein obtained from glycolysis of genistin, a kind of phytoestrogen present in soybeans, on cell proliferation and type I pN collagen synthesis in normal human dermal fibroblasts(NHDF). Cell proliferation was increased significantly with genistein treatment at 54-year aged NHDF. Genistein increased cell proliferation more strongly in cells form old doner than young doner. The senescence-associated ${\beta}$-galatosidase activity was decreased in NHDF from 77-year old doner with genistein treatment. Type I pN collagen synthesis was increased with genistein treatement in UVA treated and non-treated NHDF. The increasement of collagen synthesis was more effective in aged cells than young cells. Type I pN collagen synthesis was also increased with genistein treatment in collagen matrix culture with NHDF from sun-exposed and non-exposed skin from 54-year old doner. Genistein treatment inhibited MMP-1 synthesis in old NHDF but not in young NHDF. In conclusion, genistein may be a useful agent for preventing intrinsic aging as well as photoaging.
Keywords
Genistein; collagen; aging; photoaging; fibroblast;
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