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Effect of NaCl on Halomonas subglaciescola DH-1 Incapable of Growing at Non-Salinity  

Na, Byung-Kwan (Department of Biological Engineering, Seokyeong University)
Yoo, Young-Sun (Department of Applied Chemistry, Seokyeong University)
Park, Doo-Hyun (Department of Biological Engineering, Seokyeong University)
Publication Information
Microbiology and Biotechnology Letters / v.35, no.4, 2007 , pp. 298-303 More about this Journal
Abstract
A halophilic bacterium, H. subglaciescola DH-1, grew at 2.0 M salinity, but did not grow at 0.8 M salinity when cultivated at higher temperature ($40^{\circ}C$) than optimum ($30^{\circ}C$). When the cell extract of strain DH-1 was heated at $50^{\circ}C$ for 60 min in the absence of NaCl, isocitrate dehydrogenase and malate dehydrogenase lost their activities, but when it was heated in the presence of 2.0 M NaCl, the activity was maintained. Meanwhile, the cell extract of E. coli did not catalyze the reduction of $NAD^+$ to NADH coupled with the oxidation of isocitrate and malate at higher salinities than 1.0 M. The pH range for DH-1 was 7 to 10, and that for E. coli was 5 to 9. DH-1 was not grown in conditions with sodium salts other than NaCl.
Keywords
Halomonas subglaciescola; halophile; halotolerant; compatible solutes; alkalophile;
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