Browse > Article

Probiotic Properties of Lactobacillus salivarius CPM-7 Isolated from Chicken Feces.  

Lim, Soo-Jin (Department of Animal Resources Science, School of Bio-Resources Science, Dankook University)
Jang, Sung-Sik (Department of Animal Resources Science, School of Bio-Resources Science, Dankook University)
Kang, Dae-Kyung (Department of Animal Resources Science, School of Bio-Resources Science, Dankook University)
Publication Information
Microbiology and Biotechnology Letters / v.35, no.2, 2007 , pp. 98-103 More about this Journal
Abstract
To isolate probiotic lactic acid bacteria for animal, we have screened the microorganisms from chicken feces, by random selection and agar well diffusion assay. Among them, CPM-7 strain showing superior inhibitory activity against Escherichia coli was selected. By examining carbohydrates utilization, morphologic property and 16S rRNA gene sequence, CPM-7 strain was identified as Lactobacillus salivarius, then named L. salivarius CPM-7. L. salivarius CPM-7 produced thirteen enzymes in the test using API ZYM kit, and showed resistance to low pH and bile salts. It survived at pH 2 for 30 min. and pH 3 for 6 hr. And, it was able to grow in MRS medium containing 0.2% (w/v) bile salts. L. salivarius CPM-7 adhered to the jejunal epithelium cells of pig. Both the supernatant of L. salivarius CPM-7 and the its neutralized one showed high inhibitory activity against E. coli K88.
Keywords
L. salivarius; probiotics; antibacterial activity;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By SCOPUS : 2
연도 인용수 순위
1 Carrera-Silva, E. A., A. Silvestroni, J. G. Leblanc, J. C. Piard, G. Savoy de Giori, and F. Sesma. 2006. A thermostable alpha-galactosidase from Lactobacillus fermentum CRL 722: genetic characterization and main properties. Curr. Microbiol. 53: 374-378   DOI   ScienceOn
2 Kim, P. I., M. Y. Jung, Y. H. Chang, S. Kim., S. J. Kim, and Y. H. Park. Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract. 2006. Appl. Microbiol. Biotechnol. 74: 1103-1111   DOI
3 Mutai, M. 1983. Utilization and Effect of Latic Acid Bacteria Kor. J. Appl. Microbiol. Bioeng. 11: 339-345
4 Quwehand, A. C., P. V. Kirjavainen, C. Shortt, and S. Salminen. 1999. Probiotics: mechanisms and established effects. Int. Dairy J. 9: 43-52   DOI   ScienceOn
5 Sohn, J. H., J. H. Lee, H. Yi, J. Chun, K. S. Bae, T. Y. Ahn, and S. J. Kim. 2004. Kordia algicida gen. nov., sp. nov., an algicidal bacterium isolated from red tide. Int. J. Syst. Eviron. Microbiol. 54: 675-680   DOI   ScienceOn
6 Park, H. S., S. H. Lee, and T. B. Uhm. 1998. Selection of microorganisms for probiotics and their characterization. J. Kor. Soc. Food Sci. Nutr. 27: 433-440   과학기술학회마을
7 Gilliland, S. E., T. E. Staley, and Bush, L. J. 1984. Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjuncts. J. Dairy Sci. 67: 3045-3051   DOI   PUBMED   ScienceOn
8 Ahn, Y. T., K. L. Lim, J. C. Ryu, D. K. Kang, J. S. Ham, Y H. Jang, and H. U. Kim. 2002. Characterization of Lactobacillus acidophilus isolated from piglets and chicken. Asian-Aust. J. Anim. Sci. 15: 1790-1797   DOI
9 Ham, J. S., H. S. Kim, K. H. Hong, J. G. Kim, S. G. Jeong, H. S. Chae, J. N. Ahn, D. K. Kang, and H. U. Kim. 2003. Inhibitory activity of lactic acid bacteria against hazardous microbes. Asian-Aust. J. Anim. Sci. 16: 1550-1554   DOI
10 Mayer-Makinen, A., M. Manninen, and H. Gyllenberg. 1983. The adherence of lactic acid bacteria to the columnar epithelial cells of pigs and calves. J. Appl. Bacteriol. 55: 241-245   DOI   PUBMED
11 Fuller, R. 1989. Probiotics in man and animals. J. Appl. Bacteriol. 66: 365-378   DOI   PUBMED
12 Pavlova, S. I., A. O. Kilic, S. S. Kilic, J. S. So, M. E. Nader-Macias, J. A. Simoes, and L. Tao. 2002. Genetic diversity of vaginal Lactobacillus from women in different countries based on 16S rRNA gene sequences. J. Appl. Microbiol. 92: 451-459   DOI   ScienceOn
13 Kim, D. S. 2002. Characteristics of the bacteriocin from Lactobacillus sp. Oh-B3. Kor. J. Microbiol. Biotechnol. 30: 184-188
14 Stern, N. J., E. A. Svetoch, and B. V. Eruslanov. 2006. Isolation of a Lactobacillus salivarius strain and purification of it's bacteriocin, which is inhibitory to Campylobacter jejuni in the chicken gastrointestinal system. Antimicrob. Agents Chemother. 50: 3111-3116   DOI
15 Park, H. S., J. H. Lee, and T. B. Uhm. 1999. Probiotic properties of Lactobacillus salivarius isolated from piglet intestines. J. Kor. Soc. Food Sci. Nutr. 28: 830-836   과학기술학회마을
16 Robredo, B. and C. Torres. 2000. Bacteriocin production by Lactobacillus salivarius of animal origin. J. Clinical Microbiol. 38: 3908-3909