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Germanium-Fortified Yeast Activates Macrophage, NK Cells and B Cells and Inhibits Tumor Progression in Mice.  

Baek, Dae-Heoun (Geranti Pharm. Ltd.)
Jung, Jin-Wook (Geranti Pharm. Ltd.)
Sohn, Tsang-Uk (Geranti Pharm. Ltd.)
Kang, Jong-Koo (College of Veterinary Medicine, Chungbuk National University, Biotoxtech Co., Ltd.)
Publication Information
Microbiology and Biotechnology Letters / v.35, no.2, 2007 , pp. 118-127 More about this Journal
Abstract
Germanium-fortified yeast (GY) is a organic germanium-fortified yeast with potent immune modulating activities including anti-inflammatory effect. Through cell line studies, we observed that GY can modulate the diverse immune activity but little evidence was provided on the mechanism of GY in modulating immune activities in other higher animals. In this study, we investigated the effect of GY on modulation of immune function in mice. GY was administered in normal mice or tumor-bearing mice and then effect of GY on modulation of host immune system was analyzed by using ex vivo isolated macrophages, B cells, NK cells. Admistration of GY in mice induced macrophage activation thereby increased effector function of macrophage such as increased phagocytosis, chemotaxis, adherence, $O_2-release$, NO, $TNF-{\alpha}$ production. In addition, GY administration Increased B lymphocyte activation and plaque forming cells. Furthermore, GY administration increased NK-cell mediated cytotoxicity. Furthermore, GY administration suppressed progression of tumor in mice by increasing $TNF-{\alpha}$ production and effector function of NK cells. Our results showed that GY has a potent immunostimulatory function in vivo mice model. Proper modulation and administration of GY in human could be helpful to maintaining immunological homeostasis by modulating host immune system.
Keywords
Germanium-fortified yeast; Immunomodulator; Macrophage; NK cells and B cells; tumor suppression;
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1 Aso, H., F. Suzuki, T. Yamaguchi, Y. Hayashi, T. Ebina, and N. Ishida. 1985. Induction of interferon and activation of NK cells and macrophages in mice by oral administration of Ge-132, an organic germanium compound. Microbiol. Immunol. 29: 65-74   DOI   PUBMED
2 Bigitta, E. L., H. Mann, H. Melzer, L. Dunemann, and J. Begerow. 1999. Renal and other organ failure caused by germanium intoxication. Nephrol Dial Transplant 14: 2464-2468   DOI   ScienceOn
3 Lee, J. H, K. W. Kim, M. Y. Yoon, J. Y. Lee, C. J. Kim, and S. S. Sim. 2005. Anti-inflammatory effect of germanium-concentrated yeast against paw oedema is related to the inhibition of arachidonic acid release and prostaglandin E2 production in RBL 2H3 cells. Autonomic & Autacoid Pharmacology 25: 129-134   DOI   ScienceOn
4 Lee, J. S., J. I. Park, S. H. Kim, H. Y. Kee, Z. Z. Hwang, C. B. Park, T. U. Sohn, S. H. Shin, J. K. Kang, and Y. B. Kim. 2004. Oral single-and repeated-dose oxicity studies on Geranti Bio-Ge yeast, organic germanium fortified yeasts, in dogs. J. Toxicol. Sci. 29: 555-569   DOI   ScienceOn
5 Schauss, A. G. 1991. Nephrotoxicity and neurotoxicity in humans from organogermanium compounds and germanium dioxide. Biological Trace Element Research 29: 267-280   DOI
6 Sohn, T. U., W. J. Song, S. C. Lee, and T. K. Oh. Process for preparing S. cerevisiae containing organically bound germanium. US Pat No. 5,792,646
7 Suzuki, F. 1985. Suppression of tumor growth by isolated peritoneal macrophages from mice treated with carboxy-ethylgermanium sesquioxide (Ge-132). Japan J. Cancer Chemother. 12: 2122-2128
8 Suzuki, E, R. R. Brutkiewicz, and R. B. Pollard. 1986. Cooperation of lymphokines and macrophages in expression of antitumor activity of carboxyehtylgermanium sesquioxide (Ge-132). Anticancer Research 6: 177-182   PUBMED
9 Swenson, F. J. and T. R. Kozel. 1978. Phagocytosis of Cryptococcus neoformans by normal and thioglycolate-activated macrophages. Infection and immunity 21: 714-720   PUBMED
10 Badawi, A. M. and A. A. Hafiz. 2007. Synthesis and immunomodulatory activity of some novel amino acid germinates. J. Iran. Chem. Soc. 4: 107-113   DOI
11 Lee, S. H., K. N. Oh, S. N. Rho, B. H. Lee, and H. J. Lee. 2006. Oral repeated-dose toxicity studies especially in the liver and kidney of rats administered with organic germanium-fortified yeasts. J. Food. Sci. Nutr. 11: 115-119   과학기술학회마을   DOI
12 Okada, K., K. Okagawa, K. Kawakami, Y. Kuroda, K. Morizumi, H. Sato, H. Morita, S. Shimomura, and S. Saito. 1989. Renal railure caused by long-term use of a germanium preparation as an elixir. Clinical Nephrology 31: 219-224   PUBMED
13 Nobohiro, W., I. Osamu, K. Takuro, and Y. Koichi. 1980. New approaches to using spent brewer's yeast. ASBC J. 38: 5-8
14 Fuggetta, M. P., C. D'Onofrio, and E. Bonmassar. 1990. Role of biological response modifiers in immunochemotherapy of solid tumors and retroviral-induced leukemia. Ann Ist Super Sanita. 26: 385-395
15 Faubert, G. M. 1976. Depression of the plaque-forming cells to sheep red blood cells by the new-born larvae of Trichinella spiralis. Immunology 30: 485-489   PUBMED
16 Fukazawa, H., Y. Ohashi, S. Sekiyama, H. Hoshi, M. Abe, M. Takahashi, and T. Sato. 1994. Multidisciplinary treatment of head and neck cancer using BCG, OK-432, and GE-132 as biologic response modifiers. Head Neck. 16: 30-38   DOI   ScienceOn
17 Tao, S. H. and P. M. Bolger. 1997. Hazard assessment of germanium supplements. Regulatory Toxicology and Pharmacology 25: 211-219   DOI   ScienceOn
18 Wright, S. D. and S. C. Silverstein. 1982. Tumor promoting phorbol ester stimulated C3b and C3b' receptor-mediated phagocytosis in cultured human monocytes, J. Exp. Med. 156: 1149-1162   DOI   ScienceOn
19 Lee, S. H., S. N. Rho, and T. U. Sohn. 2005. Efficacy study of activation on macrophage in germanium-fortified yeast. J. Korean Soc. Appl. Biol. Chem. 48: 246-251   과학기술학회마을
20 Suzuki, F., R. R. Brutkiewicz, and R. B. Pollard. 1985. Importance of T-cells and macrophages in the antitumor activity of carboxyethylgermanium sesquioxide (Ge-132) Anticancer Res. 5: 479-483   PUBMED
21 Gadd, G. M. 1993. Microbial formation and transformation of organometallic and organometalloid compounds. FEMS Microbiology Reviews. 11: 297-316   DOI
22 Arimori, S., K. Watanabe, M. Yoshida, and T. Nagato. 1981. Effect of Ge-132 products and related synthetic compounds (Yamamura Y, S. Kotai, I. Azuma, A. Koda and T. Shiba. eds). Amsterdam ISBN Elsevier Science pp. 536-539
23 Goodman, S. 1988. Medical Hyphothesis; Therapeutic effects of organic germanium. 26: 207-215
24 Munakata, T., S. Arai, K. Kuwano, M. Furukawa and Y. Tomita. 1987. Induction of interferon production by NK cells by organogermanium compound, Ge-132. J. Interferon Research 7: 69-76   DOI   ScienceOn
25 Pennline, K. A. and H. B. Herscowitz. 1981. Dual role for alveolar macrophages in humoral and cell-mediated immune response: evidence for suppressor and enhancing functions. J. Reticuloendothel. Soc. 30: 205-217   PUBMED
26 Rachanee, U., H. K. Webster, K. Pattanapanxyasa, S. Pitchayangkul, and S. Thaithong. 1992. Cytoadherence characteristics of rosette-forming Plasmodium falciparum. Infection and Immunity 60: 4483-4490   PUBMED
27 Lee, S. H., S. W. Oh, S. N. Rho, B. H. Lee, H. J. Lee and D. K. Jin. 2006. Effects of germanium-fortified yeast on the serum lipids and immune cell subset. J. Korean Soc. Food Sci. Nutr. 35: 1-7   DOI
28 Joo, S. S., T. J. Won, Y. J. Lee, M. J. Kim, S. Y. Park, S. H. Lee, K. W. Hwang, and D. I. Lee. 2006. Effect of Geranti Bio-Ge yeast, a dried yeast containing biogermanium, on the production of antibodies by B cells. Immune Network 6: 86-92   과학기술학회마을   DOI
29 Lee, J. S., J. I. Park, S. H. Kim, S. H. Park, S. K. Kang, C. B. Park, T. U. Sohn, J. Y. Jang, J. K. Kang, and Y. B. Kim. 2004. Oral single-and repeated-dose oxicity studies on Geranti Bio-Ge yeast, organic germanium fortified yeasts, in rats. J. Toxicol. Sci. 29: 541-553   DOI   ScienceOn
30 Vachier, I., M. Damon, C. LeDoucen, A. C. dePaulet, P. Chanez, F. B. Michel and P. Godard. 1992. Increased oxygen species generation in blood monocytes of asthmatic patients. Am. Rev. Respir. Dis. 146: 1161-1166   DOI   PUBMED   ScienceOn