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Gene Delivery using a Novel Cationic Liposome with Low Toxicity  

Kang, Hyun-Gu (Department of Microbiology, Kyungpook National University)
Doh, Kyung-Oh (Department of Physiology, College of Medicine, Dongguk University)
Seu, Young-Bae (Department of Microbiology, Kyungpook National University)
Publication Information
Microbiology and Biotechnology Letters / v.34, no.4, 2006 , pp. 329-334 More about this Journal
Abstract
Cationic liposome has been studied as one of the most promising non-viral gene delivery systems. In this report, we describe a new synthesized cholesterolic cationic lipid (2-aminoethylcarbamate-cholesterol) and dioleylphosphatidylethanolamine (DOPE) improve the cellular uptake properties of antisense ODNs, as well as plasmid DNA with low toxicity. This formulation of cholesterolic cationic lipid termed Chol-E, efficiently transfects ODNs and plasmids into many cell types in the presense or absence of 10% serum in the medium.
Keywords
Cationic liposome; cholesterolic cationic lipid; low toxicity transfection; DOPE;
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