Browse > Article

Isolation of a Fibrinolytic Bacterium from Cheongkukjang and Characterization of Its Bioactivity  

Lee, Dong-Geun (Department of Pharmaceutical Engineering, Silla University)
Kim, Nam-Young (Department of Pharmaceutical Engineering, Silla University)
Jang, Min-Kyung (Department of Pharmaceutical Engineering, Silla University)
Yoo, Byung-Hong (Bioport Korea Corp.)
Kim, Ki-Young (Bioport Korea Corp.)
Kim, Sung-Goo (Bioport Korea Corp.)
Jeong, Yong-Kee (Department of Biotechnology, Dong-A University)
Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, Silla University)
Publication Information
Microbiology and Biotechnology Letters / v.34, no.4, 2006 , pp. 299-305 More about this Journal
Abstract
In this study, we have isolated and identified a proteolytic bacterium from conventional Cheongkukjang. We also characterized several bioactivity of Cheongkukjang, which was fermented by an isolated strain. One strain out of about $10^4$ strains obtained from Cheongkukjang showed relatively high proteolytic activity was selected and named as a Bacillus subtilis LSH805 strain. White soy-bean Cheongkukjang possessed less odor and more viscous substance than black soy-bean Cheongkukjang. Cheongkukjang showed fibrinolytic activity, and about 1,500 mg fibrin was degraded after 20 h incubation. Although nitric oxide (NO) assays of soy-bean and Cheongkukjang were almost the same, their activities were significantly higher than that of no treatment. Activity of water fraction of Cheongkukjang was somewhat higher than that of soy-bean. Fibrinolytic and NO assays of Cheongkukjang suggest that Cheongkukjang, which was fermented by an isolated strain may be a useful candidate for natural fibrinolytic and macrophage-stimulating agents.
Keywords
Bacillus subtilis; Cheongkukjang; fibrinolytic activity; nitric oxide assay;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By SCOPUS : 2
연도 인용수 순위
1 Chang J. H., Y. Y. Shim, S. H. Kim, K. M. Chee, and S. K. Cha. 2005. Fibrinolytic and immunostimulating activities of Bacillus spp. strains isolated from Chungkuk-jang. Kor. J. Food Sci. Technol. 37: 255-260   과학기술학회마을
2 Lee, D. G., M. K. Jang, N. Y. Kim, J. H. Jeon, J. H. Lee, J. H. Lee, S. J. Kim, and S. H. Lee. 2006. Screening and characterization of a novel fibrinolytic metalloproteinase from a metagenomic library. Biotechnol. Lett. in press
3 Lee, J. O., S. D. Ha, A. J. Kim, C. S. Yuh, I. S. Bang, and S. H. Park. 2005. Industrial application and physiological functions of chonggkukhang. Food Sci. Indus. 38: 69-78
4 Nathan, C. F. and J. B. Hibbs. 1991. Role of nitric oxide synthesis in macrophage antimicrobial activity. Curr. Opin. Immunol. 3: 65-70   DOI   ScienceOn
5 Ryu, S. H. 2002. Studies on antioxidative effects and antioxidative components of soybean and chongkukjang. Doctorial thesis, Inje University of Korea, 23-122
6 Wang, C. T., B. P. Ji, B. Li, R. Nout, P. L. Li, H. Ji, and L. F. Hong. 2006. Purification and characterization of a fibrinolytic enzyme of Bacillus subtilis DC33, isolated from Chinese traditional Douchi. J. Ind. Microbiol. Biotechnol. 33: 750-758   DOI
7 Woo, S. M. J. H. Kwon, and Y. J. Jeong. 2006. Selection and fermentation characteristics of Cheonggukjang strain. Kor. J. Food Preser. 13: 77-82   과학기술학회마을
8 Amber, I. J., J. B. Hibbs, R. R. Taintor, Z. Vavrin. 1988. Cytokine induce an L-arginine-dependent effector system in nonmacrophage cells. J. Leuko. Biol. 44: 58-65
9 Evans, T. G., L. Thai, D. L. Granger, and J. B. Jr. Hibbs. 1993. Effect of in vivo inhibition of nitric oxide production in murine Leishmaniasis. J. Immunol. 151: 907-915
10 Kim, S. B., D. W. Lee, C. I. Cheigh, E. A. Choe, S. J. Lee, Y. H. Hong, H. J. Choi, and Y. R. Pyun. 2006. Purification and characterization of a fibrinolytic subtilisin-like protease of Bacillus subtilis TP-6 from an Indonesian fermented soybean, Tempeh. J. Ind. Microbiol. Biotechnol. 33: 436-444   DOI
11 Shon, M. Y., S. H. Kwon, S. K. Park, and J. S. Chor. 2001. Changes in chemical components of black bean Chungkukjang added with kiwi and radish during fermentation. Kor. J. Posthavest Sci. Technol. 8: 449-455
12 Bartholomew, B. 1984. A rapid method for the assay of nitrate in urine using the nitrate reductase enzyme of Escherichia coli. Food Chem. Toxicol. 22: 541-549   DOI   ScienceOn
13 Yoo, S. M. and C. M. Chang. 1999. Study on the processing adaptability of soybean cultivars for Korean traditional chonggugjang preparation. J. Kor. Soc. Agric. Chem. Biotechnol. 42: 91-98
14 Fujita, M., K. Hong, Y. Ito, R. Fujii, K. Kariya, and S. Nishimuro. 1995. Thrombolytic effect of nattokinase on a chemically induced thrombosis model in rat. Biol. Pharm. Bull. 18: 1387-1391
15 Kim, S. S., J. H. Lee, Y. S. Ahn, J. H. Kim, and D. K. Kang. 2003. A fibrinolytic enzyme from Bacillus amyloliquefaciens D4-7 isolated from chungkook-jang; It's characterization and influence of additives on thermostability. Kor. J. Microbiol. Biotechnol. 31: 271-276
16 Green, S. J., M. S. Meltzer, J. B. Hibbs, and C. A. Nacy. 1990. Activated macrophages destroy intracellular Leishmania major amastigtes by an L-arginine-dependent killing mechanism. J. Immunol. 144: 278-283
17 Lee, S. K., S. Heo, D. H. Bae, and K. H. Choi. 1998. Medium optimization for fibrinolytic enzyme production by Bacillus subtilis KCK-7 isolated from korean traditional chungkookjang. Kor. J. Appl. Microibol. Biotechnol. 26: 226-231   과학기술학회마을
18 Anson, M. L. 1939. The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin. J. Gen. Physiol. 22: 79-85   DOI
19 Hyun, K. W., J. S. Lee, J. H. Ham, and S. Y. Choi. 2005. Isolation and identification of microorganism with potent fibrinolytic activity from Korean traditional Deonjang. Kor. J. Microbiol. Biotechnol. 33: 24-28   과학기술학회마을
20 Nakajima, N., H. Mihara, and H. Sumi. 1993. Characterization of potent fibrinolytic enzymes in earthworm, Lumbricus rubellus. Biosci. Biotechnol. Biochem. 57: 1726-1730
21 Astrup, T. and S. Mullertz. 1952. The fibrin method for estimating of fibrinolytic activity. Arch. Biochem. Biophys. 40: 346-351   DOI   ScienceOn
22 Kolb, H. and V. Kolb-Bachofen. 1992. Nitric oxide: A pathogenetic factor in autoimmunity. Immunol. Today 13: 157-160   DOI   ScienceOn
23 Snyder, S. H., D. S. Bredt. 1992. Biological roles of nitric oxide. Scientific Am. May: 68-77
24 Kim, S. C., S. H. Lee, and S. J. Wi. 2002. The effects of natto mucilage on the components of serum lipid in rats. J. Kor. Oil Chemists' Soc. 19: 63-67
25 Hibbs, J. B. Jr., Z. Vavrin, R. R. Taintor, and E. M. Rachlin. 1988. Nitric oxide: A cytotoxic activated macrophage effector molecule. Biochem. Biophys. Res. Commun. 157: 87-94   DOI   ScienceOn
26 Lee, B. Y., D. M. Kim, and K. H. Kim. 1991. Physicochemical properties of viscous substance extracted from chungkookjang. Kor. J. Food Sci. Technol. 23: 599-604
27 Kwon, H. Y., Y. S. Kim, G. S. Kwon, C. S. Kwon, and H. Y. Sohn. 2004. Isolation of immunostimulating strain Bacillus pumilus JB-1 from chungkookjang and fermentaional characteristics of JB-1. Kor. J. Microbiol. Biotechnol. 32: 291-296
28 Sumi, H., H. Hamada, K. Nakanishi, and H. Hiratani. 1990. Enhancement of the fibrinolytic activity in plasma by oral administration of nattokinase. Acta Haematol. 84: 139-143   DOI
29 Yun, G. H., E. T. Lee, and S. D. Kim. 2003. Purification and characterization of a fibrinolytic enzyme produced from Bacillus amyloliquefaciens K42 isolated from Korean soy sauce. Kor. J. Microbiol. Biotechnol. 31: 284-291   과학기술학회마을