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Immunogenicity of Recombinant Outer Membrane Protein H from Pasteurella multocida  

Lee Jeong-Min (Institute of Life Science and Technology, Sungkyunkwan University)
Publication Information
Microbiology and Biotechnology Letters / v.34, no.3, 2006 , pp. 273-277 More about this Journal
Abstract
To investigate the antigenicity and protective immunity of outer membrane protein H (OmpH) in Pasteurella multocida D:4, the recombinant OmpH protein was produced in Escherichia coli. The truncated and Trx-fused form of recombinant OmpH (53 kDa) was purified, and used as an antigen in the immunization and challenge experiment. The immunized mice with the recombinant OmpH produced a high-titer antibody, and had protective immunity against P. multocida as same level as the mice immunized with formalin-killed whole cell.
Keywords
Outer membrane protein H; Pasteurella multocida; immunogenicity; subunit vaccine;
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