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Enzyme Production Related to Alcohol Metabolism from Thermophilic Fungus Thermoascus aurantiacus  

Ko Hee-Sun (Center for Traditional Microorganism Resources, Keimyung University)
Kim Hyun-Soo (Center for Traditional Microorganism Resources, Keimyung University)
Publication Information
Microbiology and Biotechnology Letters / v.34, no.3, 2006 , pp. 216-220 More about this Journal
Abstract
Thermophillic fungus Thermoascus aurantiacus showed excellent growth and produced high amount of alcohol oxidase and catalase in a pectin medium. Besides, the strain produced enzymes which related with pectin or alcohol decomposition. We detected extracellular pectin esterase (EC 3.1.1.11) activity and, both intracellular and extracellular pectinase (EC 4.2.2.10) activity, as pectinolytic enzymes produced by T. aurantiacus. The production of methanol decomposition enzymes, such as alcohol oxidase (AOD, EC 1.1.3.13), alcohol dehydrogenase (ADH, EC 1.1.1.1), formaldehyde dehydrogenase (FADH, EC 1.2.1.1) and formate dehydrogenase (FDH, EC 1.2.1.2) follows by pectin esterase reaction which is converted to methanol. We concluded that T. aurantiacus has pectinolytic and alcohol - oxidative enzymological mechanism which produced carbon dioxide as a final material, started from pectin.
Keywords
Thermoascus aurantiacus; pectinolytic enzyme; alcohol decomposition; metabolism; catalase; alcohol oxidase;
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1 Yu, E. K. C., U. L. Tan, M. K. -H. Chan, L. Deschatelets, and N. S. John. 1987. Production of thermostable xylanase by a thermophilic fungus, Thermoascus aurantiacus. Enzyme Microb. Technol. 9: 16-24   DOI   ScienceOn
2 Ko, H., H. Fujiwara, Y. Yokoyama, N. Ohno, S. Amachi, H. Shinoyama, and T. Fujii. 2005. Inducible production of alcohol oxidase and catalase in a pectin medium by Thermoascus aurantiacus IFO 31693. J. Biosci. Bioeng. 99: 290-292   DOI   ScienceOn
3 Aebi, H. 1984. Catalase in vitro. Method Enzymol. 105: 121-126   DOI
4 Nash, T. 1953. The colorimetric estimation of formaldehyde by means of the Hantzsch reaction. Biochem. J. 55: 416-421
5 Ko, H., Y. Yokoyama, N. Ohno, M. Okadome,S. Amachi, H. Shinoyama, and T. Fujii. 2005. Purification and characterization of intracellular and extracellular, thermostable and alkali-tolerant alcohol oxidases produced by a thermophilic fungus, Thermoascus aurantiacus NBRC 31693. J. Biosci. Bioeng. 99: 290-292   DOI   ScienceOn
6 Veenhuis, M., J. P. Van Dijken, and W. Harder. 1983. The significance of peroxisomes in the metabolism of one-carbon compounds in yeasts, pp. 2-82. In A.H. Rose, J. Gareth Morris, and D. W. Tempest (ed.), Advences in microbial physiology, vol. 24, Academic Press, London
7 Wang, H., Y. Tokusige, H. Shinoyama, T. Fujii, and T. Urakami. 1998. Purification of characterization of a thermostable catalase from culture broth of Thermoascus aurantiacus. J. Ferment. Bioeng. 85: 169-173   DOI   ScienceOn
8 Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265
9 Klavons, J. A., R. D. Bennet. 1986. Determination of methanol using alcohol oxidase and its application to methyl ester content of pectins. J. Agric. Food Chem. 34: 597-599   DOI
10 Ohno, N., H. Fukuda, H. Wang, M. Kasamura, H. Shinoyama, and T. Fujii. 1998. Amylase produced by a thermophilc fungus, Thermoascus aurantiacus, and some of their properties. Seibutsu-kogaku 76: 113-119
11 Fiedurekl, J. and A. Gromada. 1997. Screening and mutagenesis of molds for improvement of simultaneous production of catalase and glucose oxidase. Enzyme Microb. Technol. 20: 344-347   DOI   ScienceOn
12 Fujii, T. and K. Tonomura. 1972. Oxidation of methanol, formaldehyde and formate by a Candida species. Agric. Biol. Chem. 36: 2297-2306
13 Khandke, K. M., P. J. Vithayathil and S. K. Murthy. 1989. Purification of xylanase, $\beta$-glucosidase, endocellulase, and exocellulase from a thermophilic fungus, Thermoascus aurantiacus. Arch. Biochem. Biophys. 274: 491-500   DOI   ScienceOn