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Optimization of Keratinolytic Protease Productions from Bacillus sp. SMMJ-2  

Park Sung-Min (Department of Microbiology, Keimyung University)
Yu Tae-Shick (Department of Microbiology, Keimyung University)
Publication Information
Microbiology and Biotechnology Letters / v.34, no.2, 2006 , pp. 150-157 More about this Journal
Abstract
Bacillus sp. SMMJ-2 producing extracellular keratinolytic protease was isolated from the Swedish soils. The optimal culture conditions for production of keratinolytic protease by Bacillus sp. SMMJ-2 were investigated. The optimal medium compositions for the keratinolytic protease production were 0.7% $K_2HPO_4$, 0.2% $KH_2PO_4$, 1.0% fructose,1.2% soybean meal (roasted), and 0.01% $Na_2CO_3$. Optimal initial pH and temperature for the production of keratinolytic protease were 7.0 and 30$^{\circ}C$, respectively. The keratinolytic protease production showed a maximum of 105 units/ml/min after 72 hours cultivation under the optimal culture conditions.
Keywords
Bacillus sp; SMMJ-2; keratinolytic protease; feather-degrading;
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