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Cloning and Characterization of Zebrafish Microsomal Epoxide Hydrolase Based on Bioinformatics  

Lee Eun-Yeol (Department of Food Science and Technology, Kyungsung University)
Kim Hee-Sook (Department of Food Science and Technology, Kyungsung University)
Publication Information
Microbiology and Biotechnology Letters / v.34, no.2, 2006 , pp. 129-135 More about this Journal
Abstract
A gene encoding for a putative microsomal epoxide hydrolase (mEH) of a zebrafish, Danio rerio, was cloned and characterized. The putative mEH protein of D. rerio exhibited sequence similarity with mammalian mEH and some other bacterial EHs. A structural model for the putative mEH was constructed using homology modeling based on the crystallographic templates, 1 qo7 and 1 ehy. The catalytic triad consisting of $Asp^{233}$, $Glu^{413}$, and $His^{440}$ was identified, and the characteristic features such as two tyrosine residues and oxyanion hole were found to be highly conserved. Based on bioinformatic analysis together with EH activity assay, the putative protein was annotated as mEH of D. rerio. Enantiopure styrene oxide with enantiopurity of 99%ee and yield of 33.5% was obtained from racemic styrene oxide by the enantioselective hydrolysis activity of recombinant mEH of D. rerio for 45 min.
Keywords
Bioinformatics; Danio rerio; microsomal epoxide hydrolase; enantiopure styrene oxide; enanti-oselective hydrolysis;
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