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Plant Growth-Promoting Capabilities of Diazotrophs from Wild Gramineous Crops  

Lee Su-Jin (Department of Microbiology, Kyung-pook National University)
Lee Sang-Eun (Department of Microbiology, Kyung-pook National University)
Seul Keyung-Jo (Department of Microbiology, Kyung-pook National University)
Park Seung-Hwan (Genome Research Center, KRIBB)
Ghim Sa-Youl (Department of Microbiology, Kyung-pook National University)
Publication Information
Microbiology and Biotechnology Letters / v.34, no.1, 2006 , pp. 78-82 More about this Journal
Abstract
Since there could be more and rather various diazotrophs in rhizosphere of wild crops than those in rhizosphere of cultivars, some wild gramineous crops grown in Korea were collected for isolating nitrogen-fixing bacteria. Six diazotrophs were purified from their roots using nitrogen-free media. The isolated bacteria were partially identified as 4 genera by 16S rDNA sequence analysis: Stenotrophomonas sp., Bosea sp., Klebsiella sp., and Azorhizobium sp. By PCR amplification and sequence analysis, DNA fragments extracted from all isolates turned out to have an individual nifH homologous gene. Five isolates (KNUC163, KNUC165, KNUC169, KNUC170, and KNUC171) showed auxin activity and four isolates (KNUC163, KNUC166, KNUC170, and KNUC171) produced siderophores. Especially,3 strains of S. maltophilia showed both auxin and siderophore activities. In conclusion, the isolated nitrogen-fixing bacteria might have capabilities for plant growth promotion.
Keywords
Nitrogen-fixing bacteria; nifH; plant growth-promoting rhizobacteria (PGPR); auxin; siderophores;
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