Browse > Article

Classification of Listeria monocytogenes Isolates from Korean Domestic Foods Using Random Amplification of Polymorphic DNA and Serotyping Analysis  

Kim Hyun-Joong (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University)
Park Si-Hong (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University)
Kim Hae-Yeong (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University)
Publication Information
Microbiology and Biotechnology Letters / v.34, no.1, 2006 , pp. 23-27 More about this Journal
Abstract
Molecular subtyping of Listeria monocytogenes, including type strains and isolates from Korean foods, were performed using random amplification of polymorphic DNA (RAPD). Each Listeria species showed specific RAPD band patterns, and L. monocytogenes serotypes and isolates were divided into two clusters. RAPD results showed that L. monocytogenes isolates from Korean foods were divided into two groups. Group I contained L. monocytogenes serotypes 1/2b and 4b, whereas Group II contained serotypes 1/2a and 1/2c. These results suggested RAPD as possible subtyping methods for Listeria species. Also, RAPD Results showed significant correlation between molecular subtyping and serotyping of L. monocytogenes, and classified two different groups of L. monocytogenes isolated from Korean foods.
Keywords
Classification; Listeria; pathogen; Random Amplification of Polymorphic DNA; serotyping;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Allerberger, F. and S. J. Fritschel. 1999. Use of automated ribotyping of Austrian Listeria monocytogenes isolates to support epidemiological typing. J. Microbiol. Meth. 35: 237-244   DOI   ScienceOn
2 Carroll, S. A., L. E. Carr, E. T. Mallison, C. Lamichanne, B. Rice, D. M. Rollins, and S. W. Joseph. 1999. Development and evaluation of a 24-hour method for the detection and quantification of Listeria monocytogenes in meat product. J. Food Proto 63: 347-353
3 Malak, M., A. Vivier, P. Andre, J. Decallonne, and P. Gilot. 2001. RAPD analysis, serotyping and esterase typing indicate that the population of Listeria monocytogenes strains recoverd from cheese and from patients with listeriosis in Belgium are different. Can. J. Microbiol. 47: 883-887   DOI
4 Nadon, C.A., D. L. Woodward, C. Young, F. G. Rodgers, and M. Wiedmann. 2001. Correlation between molecular subtyping and serotyping of Listeria monocytogenes. J. Clin. Microbiol. 39: 2704-2707   DOI   ScienceOn
5 Paillard, D., V. Dubois, R. Duran, F. Nathier, C. Guittet, P. Caumette, and C. Quentin. 2003. Rapid identification of Listeria species by using restriction fragment length polymorphism of PCR-amplified 23S rRNA gene fragments. Appl. Environ. Microbiol. 69: 6386-6392   DOI
6 Smith, M. L. and J. B. Anderson. 1989. Restriction fragment length polymorphism in mitochondrial DNAs of Armillaria. Mycolog. Res. 93: 247-256   DOI
7 Tabouret, M., J. D. Rycke, and G. Dubray. 1992. Analysis of surface proteins of Listeria in relation to species, serovar and pathogenicity. J. Gen. Microbiol. 138: 743-753   DOI
8 Volokhov, D., A. Rasooly, K. Chummakov, and V. Chizhikov. 2002. Identification of Listeria species by Microarray-based assay. J. Clin. Microbiol. 40: 4720-4728   DOI
9 Wiedmann, M., J. L. Bruce, C. Keating, A. E. Johnson, P. L. Mcdonough, and C. A. Batt. 1997. Ribotypes and virulence gene polymorphisms suggest three distinct Listeria monocytogenes lineages with differences in pathogenic potential. Infect. Immun. 65: 2707-2716
10 Mereghetti, L., P. Lanotte, V. N. Savoye-Marczuk, A. Audurier, and R. Quentin. 2002. Combined ribotyping and random multiprimer DNA analysis to probe the population structure of Listeria monocytogenes. Appl. Environ. Microbiol. 68: 2849-2856   DOI
11 Rasmussen, O. F., P. Skouboe, L. Dons, L. Rossen, and I.E. Olsen. 1995. Listeria monocytogenes exists in at least three evolutionary lines: evidence from flagellin, invasive associated protein and listeriolysin a genes. Microbiology 141: 2053-2061   DOI   ScienceOn
12 Guerra, M. M., F. Bernardo, and J. Mclauchlin. 2002. Amplified Fragment length polymorphism (AFLP) analysis of Listeria monocytogenes. System. Appl. Microbiol. 25: 456-461   DOI   ScienceOn
13 Martinez, J., L. M. Rorvik, V. Brox, J. Lassen, M. Seppola, L. Gram, and B. Fonnesbech-Vogel. 2003. Genetic variability among isolate of Listeria monocytogenes from food products, clinical samples and processing environment, estimated by RAPD typing. Int. J. Food Microbiol. 84: 285-297   DOI   ScienceOn
14 Norrung, B. and N. Skovgaard. 1993. Application of multilocus enzyme electrophoresis in studies of the epidemiology of Listeria monocytogenes in Denmark. Appl. Environ. Microbiol. 59: 2817-2822
15 Byun, S. K., S. C. Jung, and H. S. Yoo. 2001. Random amplification of polymorphic DNA typing of Listeria monocytogenes isolated from meat. Int. J. Food Microbiol. 69: 227-235   DOI   ScienceOn