Browse > Article

Cloning and Characterization of the Paraquat Resistance-Related Genes from Ochrobactrum anthropi JW-2  

Bae Eun-Kyung (Biotechnology Division, Korea Forest Research Institute)
Lee Hyo-Shin (Biotechnology Division, Korea Forest Research Institute)
Won Sung-Hye (Division of Animal Science and Technology, College of Agriculture and Life Science, Gyeongsang National University)
Lee Byung-Hyun (Division of Animal Science and Technology, College of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Microbiology and Biotechnology Letters / v.34, no.1, 2006 , pp. 15-22 More about this Journal
Abstract
A 4,971 bp chromosomal DNA fragment containing the pqrA, paraquat resistance gene, was cloned from Ochrobactrum anthropi JW-2, and the complete nucleotide sequence was determined. Nucleotide and deduced amino acid sequences of the fragment revealed the presence of 4 complete ORFs (orf2, pqrA, orf3, orf4) and two incomplete ORFs(orf1, orf5). Orf1, pqrA, orf4 and orf5 exists at the direct strand but orf2 and orf3 exists at the reverse complementary strand. Orf1 which of incomplete sequences without start codon shares homology with ATP binding region of the response regulator receiver. Orf2 shares high homology with members of the tetR family of transcriptional repressor which have a helix-turn-helix (H-T-H) motif. Therefore, the orf2 is predicted as a transcriptional repressor of pqrA and is designated as pqrR2. Orf3 shares high homology with the members of the lysR family acting as a transcriptional activator which have both of a H-T-H motif at the N-terminal region and substrate binding domain at the C-terminal region. Therefore, the orf3 is predicted as a transcriptional activator of pqrA and is designated as pqrR1. Orf4 shows homology with the periplasmic substrate-binding protein of amino acid ABC transporter. Orf5 which of incomplete sequences without stop codon revealed the homology with the permeases protein of amino acid ABC transporter.
Keywords
Paraquat; paraquat resistance-related genes; Ochrobactrum anthropi JW-2;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Cho, Y. H., E. J. Kim, H. J. Chung, J. H Choi, K. F. Chater, B. E. Ahn, J. H. Shin, and J. H. Roe. 2003. The pqrAB operon is responsible for paraquat resistance in Streptomyces coelicolor. J. Bacteriol. 185: 6756-6763
2 Comai L., D. Facciotti, W. R. Hiatt, G. Thompson, R. E. Rosi, and D. M. Stalker. 1985. Expression in plants of mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317: 741-744   DOI
3 Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166: 557-580   DOI
4 Kowallik, K. v., B. Stoebe, J. Schaffran, P. Kroth-Pancic, and U. Freier. 1995. The chloroplast genome of a chlorophyll a+c-containing alga, Odontella sinensis. Plant Mol. Biol. Rep. 13: 336-342   DOI
5 Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463-5467
6 Stalker, D. M., W. R. Hiatt, and L. Comai. 1985. A single amino acid substitution in the enzyme 5-enolpyruvylshikimate-3-phosphate synthase confers resistance to the herbicide glyphosate. J. BioI Chern. 260: 4724-4728
7 Windhovel, D. and B. Bowine. 1991. Identification of cfxR, an activator gene of autotrophic CO2 fixation in Alcaligenes eutrophus. Mol. Micobiol. 5: 2695-2705   DOI
8 Won, S. H., B. H. Lee, H. S. Lee, and J. K. Jo. 2001. An Ochrobactrum anthropi gene eonferring paraquat resistance to the heterologous host Escherichia coli. Biochem. Biophys. Res. Comm. 285: 885-890   DOI   ScienceOn
9 Murgia, I., D. Tarantino, C. Vannini, M. Bracale, S. Carravieri, and C. Soave. 2004. Arabidopsis thaliana plants overexpressing thylakoidal ascorbate peroxidase show increased resistance to Paraquat-induced photooxidative stress and to nitric oxide-induced cell death. Plant J. 38: 940-953   DOI   ScienceOn
10 Sambrook, J. and D.W. Russell. 2001. Molecular cloning: a laborotory manual, 3rd (eds.), p. 116. Cold spring Harbor Laboratory Press, Cold Spring Harbor, N.Y
11 Kerppola, R. E. and G. Ferro-Luzzi Ames. 1992. Topology of the hydrophobic membrane-bound components of the histidine preiplasmic permease. J. Biol. Chem. 267: 2329-2336
12 Preston, C. 1994. Resistance to photosystem I disrupting herbicide. p. 61-82. In S. B. Powles and A. M. Holtum (eds.), Herbicide Resistance in plants: Biology and Biochemistry. Lewis Publishers, Boca Raton, FL
13 Santiviago, C. A., J. A. Fuentes, S. M. Bueno, A. N. Trombert, A. A. Hildago, L. T. Socias, P. Youderian, and G. C. Mora. 2002. The Salmonella enterica sv. Typhimurium smvA, yddG and ompD (porin) genes are required for the efficient efflux of methyl viologen. Mol. Microbiol. 46: 687-698   DOI   ScienceOn
14 Muraoka, S., R. Okumura, N. Ogawa, T. Nonaka, K. Miyashita, and T. Senda. 2003. Crystal structure of a fulllength lysR-type transcriptional regulator, CbnR: Unusual combination of two subunit forms and molecular bases for causing and changing DNA bend. J. Mol. Biol. 328: 555-566   DOI   ScienceOn
15 Schumacher, M. A., M. C. Miller, S. Grkovic, M. H. Brown, R. A. Skurray, and R. G. Brennan. 2001. Structural mechanisms of QacR induction and multidrug recognition. Science 294: 2158-2163   DOI   ScienceOn
16 Kim, S. E., S. Y. Kim, S. H. Ahn, and J. C. Chun. 2005. Determination of paraquat-resistant biotype on Conyza canadensis and the resistant mechanism. Kor. J. Pesticide Sci. 9: 88-96   과학기술학회마을
17 Viale, A. M., H. Kobayashi, T. Akazawa, and S. Henikoff. 1991. RbcR [correction of rcbR], a gene coding for a member of the LysR family of transcriptional regulators, is located upstream of the expressed set of ribulose 1,5-bisphosphate carboxylase/oxygenase genes in the photosynthetic bacterium Chrornatiurn vinosurn. J. Bacteriol. 173: 5224-5229   DOI
18 Stragier, P., O. Danos, and J. C. Patte. 1983. Regulation of diaminopimelate decarboxylase synthesis in Escherichia Coli. II. Nucleotide sequence of the lysA gene and its regulatory region. J. Mol. Biol. 168: 321-331   DOI
19 Jo, J. K., S. H. Won, D. Y. Son, and B. H. Lee. 2004. Paraquat resistance of transgenic tobacco plants overexpressing the Ochrobactrum anthorpi pqrA gene. Biotechnol. Lett. 26: 1391-1396   DOI   ScienceOn
20 Yu, T., Y. S. Li, X. F. Chen, J. H. Hu, X. C. Chang, and Y. G. Zhu. 2003. Transgenic tobacco plants overexpressing cotton glutathione S-transferase (GST) show enhanced resistance to methyl viologen. J. Plant Physiol. 160: 1305-131l   DOI   ScienceOn
21 Wissenbach, D., S. Six, J. Bongaerts, D. Ternes, S. Steinwachs, and G. Dnden. 1995. A third periplasmic transport system for L-arginine in Eschericia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport. Mol. Microbiol. 17: 675-686   DOI   ScienceOn
22 Stalker, D. M., K. E. McBride, and L. D. Malyj. 1988. Herbicide resistance in transgenic plants expressing a bacterial detoxification gene. Science 242: 419   DOI
23 Won, S. H., B. H. Lee, and J. K. Jo. 2000. Characterization of a paraquat resistance of Ochrobactrum anthroi JW-2. Kor. J. Appl. Microbiol. Biotechnol. 28: 1-7
24 Ye, B. and J. Cressel. 2000. Transient, oxidant-induced antioxidant transcript and enzyme levels correlate with greater oxidant-resistance in paraquat-resistant Conyza bonariensis. Planta 211: 50-61   DOI
25 Barry, G. F., M. L. Taylor, S. R. Padgette, K. H. Kolacz, L. E. Hallas, G. Della-Cioppa, and G. M. Kishore. 1994. Cloning and expression in Escherichia coli of the glyphosate-toaminomethyl phos-phonic acid degrading activity from Achromobacter sp. strain LBAA. Monsanto Technical Report MSLB 13245, St. Louis