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Cultural Conditions for Pretense Production by a feather-Degrading Bacterium, Bacillus megaterium F7-1  

Son Hong-Joo (Department of Biotechnology, Miryang National University)
Publication Information
Microbiology and Biotechnology Letters / v.33, no.4, 2005 , pp. 315-318 More about this Journal
Abstract
The effects of inorganic salts and feather concentrations on pretense production by Bacillus megaterium F7-1 were investigated. Pretense production was dependent on the presence of phosphates in the medium. Supplementation of medium with calcium ion slightly increased protease production. The highest protease production was obtained at $1.4\%$ feather. The optimal medium contained $2.0\%$ glucose, $0.8\%$ skim milk, $0.06\%\;K_{2}HPO_{4}\%,\;0.04\%\;KH_{2}PO{4},\;0.06\%\;NaCl,\;0.03\%\;MgCl_{2}\cdot6H_{2}O,\;0.002\%\;CaCl_{2}\cdot2H_{2}O,\;and\;1.4\%$ whole feather. By using this optimized medium, increased production of the protease was achieved compared with the cases of using basal medium.
Keywords
Bacillus megaterium; feather; protease;
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1 Cheng, S. W., H. M. Hu, S. H. Shen, H. Takai, M. Asano, and Y. C. Tsai. 1995. Production and characterization of keratinase of a feather-degrading Bacillus licheniformis PWD-J. Biosci. Biochem. Biotechnol. 59: 2239-2243   DOI   ScienceOn
2 Kim, Y. B., J. B. Lee, K. S. Sung, and N. H. Lee. 1998. Effects of physical processing on protein content and pepsindigestibility offeather meals. Kor. J. Anim. Sci. 40: 103-110
3 Ramnani, P. and R. Gupta. 2004. Optimization of medium composition for keratinase production on feather by Bacillus licheniformis RGI using statistical methods involving response surface methodology. Biotechnol. Appl. Biochem. 40: 191-196   DOI   ScienceOn
4 Onifade, A. A., N. A. AI-Sane, A. A. AI-Musallam, and S. AI-Zarban. 1998. A review: potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Bioresource Technol. 66: 1-11   DOI   ScienceOn
5 Son, H. J. G T. Park, and Y. G Kim. 2004. Production of a keratinolytic protease by a feather-degrading bacterium, Bacillus megaterium F7-1. Kor. J. Microbiol. 40: 43-48
6 Lahl, W. J. and S. D. Braun. 1994. Enzymatic production of protein hydrolysates for food use. Food Technol. October 68-71
7 Charney, J. and R. M. Tomarelli. 1947. A colourimetric method for the determination of proteolytic activity of duodenal juice. J. Biol. Chem. 171: 501-505
8 Son, H. J., Y. G Kim, and Y. K. Park. 2004. Isolation and identification of feather-degrading bacteria for biotechnological applications of keratinaceous protein waste. J. Life Sci. 14: 229-234   DOI
9 EI-Refai, H. A., M. A. AbdelNaby, A. Gaballa, M. H. EIAraby, and A. F. Fattah. 2005. Improvement of the newly isolated Bacillus pumilis FH9 keratinolytic activity. Process Biochem. 40: 2325-2332   DOI   ScienceOn
10 Lin, X., C. C. Lee, E. S. Casale, and J. C. H. Shih. 1992. Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain. Appl. Environ. Microbiol. 58: 3271-3275   PUBMED
11 Rao, M. B., A. M. Tanksale, M. S. Ghatge, and V. V. Deshpande. 1998. Molecular and biotechnological aspects of microbial proteases. Microbiol. Mol. Biol. Rev. 62: 597-635   PUBMED
12 Papadopoulos, M. C., A. R. EI Boushy, and A. E. Roodbeen. 1985. The effect of varying autoclaving conditions and added sodium hydroxide on amino acid content and nitrogen characteristics of feather meal. J. Sci. Food Agric. Abstr. 36: 1219-1226   DOI
13 Singh, C. J. 2002. Optimization of an extracellular protease of Chrysosporium keratinophilum and its potential in bioremediation of keratinic wastes. Mycophathologia 156: 151-156
14 Korea Chicken Council. 2002. www.chicken.or.kr/3data/do.htm
15 Oyeka, C. A. and H. C. Gugnani. 1997. Keratin degradation by Scytalidium species and Fusarium solani. Mycoses 41: 73-76
16 Papadopoulos, M. C. 1985. Processed chicken feathers as feedstuff for poultry and swine. A review. Agric. Wastes 14: 275-290   DOI